Digoxin

别名: NSC 95100, 12β-Hydroxydigitoxin 中文名称:地高辛

Digoxin是一种经典的Na,K-ATPase抑制剂,其对α2β3亚型的选择性高于α1β1亚型,用于治疗心房颤动和心脏衰竭。此产品为危化品(急性毒性/易燃/皮肤腐蚀),请在穿戴防护面罩、防护手套和防护服后使用。 已取得危险化学品经营许可

Digoxin Chemical Structure

Digoxin Chemical Structure

CAS: 20830-75-5

规格 价格 库存 购买数量
100mg RMB 801.45 现货
500mg RMB 1599.79 现货
更大包装 有超大折扣

400-668-6834

info@selleck.cn

免费分装
免费预溶

客户使用Selleck的发表文献12

产品质控

批次: 纯度: 99.61%
99

Digoxin相关产品

相关信号通路图

ATPase抑制剂选择性比较

细胞实验数据示例

细胞系 实验类型 给药浓度 孵育时间 活性描述 文献信息
Hs578Bst Cytotoxicity assay 40 uM 72 hrs Cytotoxicity against human Hs578Bst cells at 40 uM after 72 hrs by CellTiter-Glo assay relative to untreated control, IC50=47.7μM 22316168
HeLa Function assay 150 nM 24 hrs Inhibition of Na(+)/K(+) ATPase in human HeLa cells at 150 nM after 24 hrs by immunoblotting 26122772
HeLa Function assay 150 nM 24 hrs Inhibition of Na(+)/K(+) ATPase in human HeLa cells at 150 nM after 24 hrs by immunoblotting 26122772
HeLa Function assay 150 nM 24 hrs Inhibition of Na(+)/K(+) ATPase in human HeLa cells at 150 nM after 24 hrs by immunoblotting 26122772
HeLa Function assay 150 nM 24 hrs Inhibition of Na(+)/K(+) ATPase in human HeLa cells at 150 nM after 24 hrs by immunoblotting 26122772
HeLa Function assay 150 nM 24 hrs Inhibition of Na(+)/K(+) ATPase in human HeLa cells at 150 nM after 24 hrs by immunoblotting 26122772
HeLa Function assay 150 nM 24 hrs Inhibition of Na(+)/K(+) ATPase in human HeLa cells at 150 nM after 24 hrs by immunoblotting 26122772
HeLa Function assay 150 nM 24 hrs Inhibition of Na(+)/K(+) ATPase in human HeLa cells at 150 nM after 24 hrs by immunoblotting 26122772
HeLa Function assay 150 nM 24 hrs Inhibition of Na(+)/K(+) ATPase in human HeLa cells at 150 nM after 24 hrs by immunoblotting 26122772
DU145 Function assay 50 uM 5 hrs Inhibition of Na+/K+ ATPase pump in human DU145 cells assessed as increase in intracellular calcium level at 50 uM measured after 5 hrs by Fura-2/AM dye based fluorescence assay 31120251
DU145 Function assay 50 uM 5 hrs Inhibition of Na+/K+ ATPase pump in human DU145 cells assessed as increase in intracellular calcium level at 50 uM measured after 5 hrs by Fura-2/AM dye based fluorescence assay 31120251
DU145 Function assay 50 uM 5 hrs Inhibition of Na+/K+ ATPase pump in human DU145 cells assessed as increase in intracellular calcium level at 50 uM measured after 5 hrs by Fura-2/AM dye based fluorescence assay 31120251
DU145 Function assay 50 uM 5 hrs Inhibition of Na+/K+ ATPase pump in human DU145 cells assessed as increase in intracellular calcium level at 50 uM measured after 5 hrs by Fura-2/AM dye based fluorescence assay 31120251
DU145 Function assay 50 uM 5 hrs Inhibition of Na+/K+ ATPase pump in human DU145 cells assessed as increase in intracellular calcium level at 50 uM measured after 5 hrs by Fura-2/AM dye based fluorescence assay 31120251
DU145 Function assay 50 uM 5 hrs Inhibition of Na+/K+ ATPase pump in human DU145 cells assessed as increase in intracellular calcium level at 50 uM measured after 5 hrs by Fura-2/AM dye based fluorescence assay 31120251
DU145 Function assay 50 uM 5 hrs Inhibition of Na+/K+ ATPase pump in human DU145 cells assessed as increase in intracellular calcium level at 50 uM measured after 5 hrs by Fura-2/AM dye based fluorescence assay 31120251
DU145 Function assay 50 uM 5 hrs Inhibition of Na+/K+ ATPase pump in human DU145 cells assessed as increase in intracellular calcium level at 50 uM measured after 5 hrs by Fura-2/AM dye based fluorescence assay 31120251
human hepatocytes Function assay 0.05 to 10 uM Drug uptake in sandwich-cultured human hepatocytes at 0.05 to 10 uM at 37 degC by Michaelis-Menten kinetics/scintillation spectroscopy, Km=2.39 μM 20926619
HCT116 Cytotoxicity assay 72 hrs Cytotoxicity against human HCT116 cells after 72 hrs by FMCA method, IC50=0.27μM 19894733
CC20 Cytotoxicity assay 72 hrs Cytotoxicity against human CC20 cells after 72 hrs by FMCA method, IC50=0.24μM 19894733
colon cancer Cytotoxicity assay 72 hrs Cytotoxicity against human colon cancer cells after 72 hrs by FMCA method, IC50=0.1μM 19894733
UACC62 Cytotoxicity assay 48 hrs Cytotoxicity against human UACC62 cells after 48 hrs by SRB assay, IC50=0.0295μM 16309315
MCF7 Cytotoxicity assay 48 hrs Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay, IC50=0.0241μM 16309315
TK10 Cytotoxicity assay 48 hrs Cytotoxicity against human TK10 cells after 48 hrs by SRB assay, IC50=0.0146μM 16309315
HT-29 Cytotoxicity assay 72 hrs Cytotoxicity against human HT-29 cells after 72 hrs by FMCA method, IC50=1.4μM 19894733
Hs578Bst Cytotoxicity assay 72 hrs Cytotoxicity against human Hs578Bst cells after 72 hrs by CellTiter-Glo assay, IC50=0.04μM 22316168
Hs578T Cytotoxicity assay 72 hrs Cytotoxicity against human Hs578T cells after 72 hrs by CellTiter-Glo assay, IC50=0.251μM 22316168
T47D Cytotoxicity assay 72 hrs Cytotoxicity against human T47D cells after 72 hrs by CellTiter-Glo assay, IC50=0.66μM 22316168
SK-BR-3 Cytotoxicity assay 72 hrs Cytotoxicity against human SK-BR-3 cells after 72 hrs by CellTiter-Glo assay, IC50=1.08μM 22316168
MCF7 Cytotoxicity assay 72 hrs Cytotoxicity against human MCF7 cells after 72 hrs by CellTiter-Glo assay, IC50=4.7μM 22316168
Hs683 Cytotoxicity assay 3 days Cytotoxicity against human Hs683 cells after 3 days by MTT assay, IC50=0.04μM 23706005
A549 Cytotoxicity assay 3 days Cytotoxicity against human A549 cells after 3 days by MTT assay, IC50=0.05μM 23706005
PC3 Cytotoxicity assay 3 days Cytotoxicity against human PC3 cells after 3 days by MTT assay, IC50=0.075μM 23706005
U373 Cytotoxicity assay 3 days Cytotoxicity against human U373 cells after 3 days by MTT assay, IC50=0.146μM 23706005
SK-MEL-28 Cytotoxicity assay 3 days Cytotoxicity against human SK-MEL-28 cells after 3 days by MTT assay, IC50=0.266μM 23706005
MCF7 Cytotoxicity assay 3 days Cytotoxicity against human MCF7 cells after 3 days by MTT assay, IC50=0.426μM 23706005
RKO-AS45-1 Cytotoxicity assay 48 hrs Cytotoxicity against human RKO-AS45-1 cells after 48 hrs by MTT assay, IC50=0.42μM 26122772
HeLa Cytotoxicity assay 48 hrs Cytotoxicity against human HeLa cells after 48 hrs by MTT assay, IC50=2.2μM 26122772
Kasumi-1 Cytotoxicity assay 48 hrs Cytotoxicity against human Kasumi-1 cells incubated for 48 hrs by MTS assay, IC50=0.093μM 27983842
MV4-11 Cytotoxicity assay 48 hrs Cytotoxicity against human MV4-11 cells incubated for 48 hrs by MTS assay, IC50=0.111μM 27983842
HT-29 Cytotoxicity assay 72 hrs Cytotoxicity against human HT-29 cells incubated for 72 hrs by SRB assay, IC50=0.38μM 27983842
H1299 Cytotoxicity assay 24 hrs Cytotoxicity against human H1299 cells incubated for 24 hrs by MTT assay, IC50=0.46μM 27983842
CCD-112CoN Cytotoxicity assay 72 hrs Cytotoxicity against human CCD-112CoN cells incubated for 72 hrs, IC50=2.4μM 27983842
HeLa Function assay Inhibition of mitosis in human HeLa cells by imaging analysis 18066055
HeLa Cytotoxicity assay Cytotoxicity against human HeLa cells assessed as inhibition of DNA replication by imaging analysis 18066055
K562 Cytotoxicity assay Cytotoxicity against human K562 cells by XTT assay, IC50=0.0282μM 16309315
MDCK Function assay TP_TRANSPORTER: cell accumulation in OATP4C1-expressing MDCK cells, Km=8μM 14993604
MDCK Function assay TP_TRANSPORTER: cell accumulation in OATP4C1-expressing MDCK cells, Km=7.8μM 14993604
LLC-PK1 Function assay TP_TRANSPORTER: uptake in Oatp2-expressing LLC-PK1 cells, Km=0.279μM 11883641
LLC-PK1 Function assay TP_TRANSPORTER: uptake in Oatp2-expressing LLC-PK1 cells, Km=1.07μM 11792694
LLC-PK1 Function assay TP_TRANSPORTER: inhibition of Taurocholate uptake in Oatp2-expressing LLC-PK1 cells, Ki=0.58μM 11792694
High Five (BTI-TN5B1-4) Function assay TP_TRANSPORTER: ATP hydrolysis in membrane fraction from High Five (BTI-TN5B1-4) cells, Km=25.9μM 11785684
LLC-PK1 Function assay TP_TRANSPORTER: inhibition of E217betaG uptake in Oatp2-expressing LLC-PK1 cells, Ki=0.037μM 11408557
rat hepatocytes Function assay Drug uptake in rat hepatocytes, Km=0.18 μM 20926619
CHO Function assay pIC50 values for sodium fluorescein (10 uM) uptake in OATP1B1-transfected CHO cells, IC50=3.23594μM 23571415
CHO Function assay pIC50 values for sodium fluorescein (10 uM) uptake in OATP1B3-transfected CHO cells, IC50=13.1826μM 23571415
Drosophila Schneider cells Function assay Antagonist activity at transactivation domain of RORgammat (unknown origin) expressed in Drosophila Schneider cells co-expressing Gal4-DNA binding domain, IC50=2μM 24040486
Drosophila Schneider cells Function assay Antagonist activity at transactivation domain of RORgammat (unknown origin) expressed in Drosophila Schneider cells co-expressing Gal4-DNA binding domain, IC50=2μM 24040486
点击查看更多细胞系数据

生物活性

产品描述 Digoxin是一种经典的Na,K-ATPase抑制剂,其对α2β3亚型的选择性高于α1β1亚型,用于治疗心房颤动和心脏衰竭。此产品为危化品(急性毒性/易燃/皮肤腐蚀),请在穿戴防护面罩、防护手套和防护服后使用。
靶点
Na,K-ATPase α2β3 [1] Na,K-ATPase α2β1 [1] Na,K-ATPase α1β1 [1]
42.8 nM(Ki) 58.7 nM(Ki) 268 nM(Ki)

化学信息&溶解度

分子量 780.94 分子式

C41H64O14

CAS号 20830-75-5 SDF Download Digoxin SDF
Smiles CC1C(C(CC(O1)OC2C(OC(CC2O)OC3C(OC(CC3O)OC4CCC5(C(C4)CCC6C5CC(C7(C6(CCC7C8=CC(=O)OC8)O)C)O)C)C)C)O)O
储存条件(自收到货起)

体外溶解度
批次:

DMSO : 100 mg/mL ( 128.05 mM; DMSO吸湿会降低化合物溶解度,请使用新开封DMSO)

Water : Insoluble

Ethanol : Insoluble

摩尔浓度计算器

体内溶解度
批次:

现配现用,请按从左到右的顺序依次添加,澄清后再加入下一溶剂

动物体内配方计算器

实验计算

摩尔浓度计算器

质量 浓度 体积 分子量

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

* 必填项

请输入您的姓名
请输入您的邮箱地址 请输入一个有效的邮箱地址
请写点东西给我们
Tags: buy Digoxin | Digoxin supplier | purchase Digoxin | Digoxin cost | Digoxin manufacturer | order Digoxin | Digoxin distributor
在线咨询
联系我们