Proteasome 20S LMP7 Rabbit Recombinant mAb

生物描述

特异性

Proteasome 20S LMP7 Rabbit Recombinant mAb detects endogenous level of total Proteasome 20S LMP7.

背景

The immunoproteasome is an alternative form of the constitutive proteasome that is a key component of the proteolytic machinery. When the cells are stimulated by inflammatory stimuli, such as interferon-γ or TNF-α, three subunits of the constitutive proteasome β1, β2, and β5 are replaced with the immunoproteasome catalytic subunits β1i (low-molecular mass protein [LMP] 2), β2i (multicatalytic endopeptidase complex-like [MECL] 1), and β5i (LMP7), respectively. LMP7 is required to produce proinflammatory cytokines, such as TNF-α and IL-6, and to progress experimental arthritis and colitis. LMP7 is predominantly expressed in immune and inflammatory cells. Immunoproteasome has diverse functions such as regulation of key processes in maintaining cellular homeostasis and in responding to stress other than antigen presentation.

使用信息

抗体应用	WB, IHC,ELISA,ELISA
稀释比例	WB IHC 1:1000 1:50
反应性	Human Mouse Rat
MW (kDa)	23kDa
抗体类型	Rabbit
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Hiroaki Kimura, et al. Scientific Reports. 2015, 5, Article number: 15883. [2] Deborah A. Ferrington, et al. Prog Mol Biol Transl Sci. 2012, 109: 75-112.

Application Data

WB

IHC