14-3-3 alpha/beta Rabbit Recombinant mAb

生物描述

特异性

14-3-3 alpha/beta Rabbit Recombinant mAb detects endogenous level of total 14-3-3 alpha/beta.

背景

The 14-3-3 proteins are a family of conserved regulatory molecules expressed in all eukaryotic cells. They could bind a multitude of functionally diverse signaling proteins, including kinases, phosphatases, and transmembrane receptors and plays important roles in a wide range of vital regulatory processes, such as mitogenic signal transduction, apoptotic cell death, and cell cycle control. 14-3-3 is present in almost all tissues, including testes, liver, and heart. Within a eukaryotic cell, 14-3-3 is largely found in the cytoplasmic compartment. However, 14-3-3 proteins can also be detected at the plasma membrane and in intracellular organelles such as the nucleus and the Golgi apparatus. 14-3-3 protein beta/alpha may be a diagnostically useful biomarker for early diagnosis of renal cell carcinoma.

使用信息

抗体应用	WB, IHC, IF,ELISA,ELISA
稀释比例	WB IHC IF 1:1000 1:50 1:50
反应性	Human Mouse Rat
MW (kDa)	28kDa
抗体类型	Rabbit
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Fu H, et al. Annu Rev Pharmacol Toxicol. 2000, 40:617-47. [2] Minamida S, et al. Anal Bioanal Chem. 2011, 401(1):245-52.

Application Data

WB

IHC

IF