Phospho-Histone H1.4 (T17) Rabbit Recombinant mAb

生物描述

特异性

Phospho-Histone H1.4 (T17) Rabbit Recombinant mAb detects endogenous level of total Phospho-Histone H1.4 (T17).

背景

The linker histone H1 binds to the short stretches of DNA that link individual nucleosomes and, thereby, establishes and stabilises the chromatin fibre. Similar to other histones, histone H1 can be modified by phosphorylation, methylation, acetylation and ADP-ribosylation. Phosphorylation of histone H1.4 on S27 by Aurora B kinase has an important regulatory role in epigenetic regulation, chromatin structure and function. H1.4 is a new mitotic marker and show that levels of S27 phosphorylation are highest during metaphase. Phosphorylated H1.4 binds to mitotic chromatin with an affinity that is higher than that of the unphosphorylated form, suggesting a role for S27 phosphorylation in H1.4 mobility and chromatin binding.

使用信息

抗体应用	WB, IF,ELISA,ELISA
稀释比例	WB IF 1:1000 1:50
反应性	Human Mouse Rat
MW (kDa)	30kDa
抗体类型	Rabbit
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] New code for histone H1.4. J Cell Sci. 2011, 124: e1003.

Application Data

WB

IF