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特异性 | Phospho-Smad5 (S463+S465) Rabbit Recombinant mAb detects endogenous level of total Phospho-Smad5 (S463+S465). |
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背景 | Smads are signal transducers for the members of the transforming growth factor-beta (TGF-beta) superfamily. Smads are subdivided into three subclasses based on their structure and function. Receptor-regulated Smads (R-Smads) have a C-terminal Ser-Ser-X-Ser motif, and are directly phosphorylated by the type I receptor kinases. R-Smads then form complexes with common-partner Smads (Co-Smads) and translocate into the nucleus, where they regulate the transcription of target genes. Inhibitory Smads (I-Smads) are induced by ligand stimulation and interfere with the receptor activation or complex formation of R-Smads. Upon BMP or TGFβ stimulation, cytosolic R-Smad members, including Smad1, Smad2, Smad3, Smad5 and Smad8, become phosphorylated by the type I receptors at the most C-terminal SSXS motif. Upon BMP or TGFβ stimulation, cytosolic R-Smad members, including Smad1, Smad2, Smad3, Smad5 and Smad8, become phosphorylated by the type I receptors at the most C-terminal SSXS motif. Although amino acid sequences of R-Smads share extremely high homology, Smad1/5/8 favor BMP signaling, while Smad2/3 mainly act downstream of the TGFβ pathway. |
抗体应用 | WB ,ELISA,ELISA | ||
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稀释比例 |
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反应性 | Human Mouse Rat | ||
MW (kDa) | 58kDa | ||
抗体类型 | Rabbit | ||
储存液配方 | 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. | ||
储存条件 (自收到货起) |
Store at –20°C. |
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WB
Validated by Selleck