AF647 Human/mouse/rat β-Tubulin Antibody [9F3]

目录号：G5097

打印

生物描述

特异性
背景 Beta-微管蛋白与 alpha-微管蛋白结合形成异源二聚体，是微管的基本亚基，微管是真核细胞中构成细胞骨架的三种主要胞质纤维之一。与微丝（肌动蛋白丝）和中间丝一起，微管提供关键的结构支持，并对众多细胞过程至关重要。这些微管蛋白亚基聚合成微管聚合物的过程需要 gamma-微管蛋白的参与，它负责成核和组织组装过程。

使用信息

抗体应用 FCM, IHC, IF 推荐用量 0.2 μg ( 2.5 μL) / million cells in 100 µL volume
反应性 Human, Mouse, Rat, Monkey, Zebrafish, Bovine, Chicken
抗体类型 Rabbit MW 55 kDa
储存液配方 Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
储存条件
(自收到货起)		 Store at 4°C in the dark to avoid freeze-thaw cycles

Application Data

FCM

Validated by Selleck

  • Fixation of Jurkat cells with 4% formaldehyde, after washing, cells were permeabilized by adding 1000 μL of pre-cooled 90% methanol, followed by washing, and then staining of cells using AF647 Human/mouse/rat β-Tubulin Antibody [9F3] (Selleck Cat.: G5097) (green, with red blank control). Analyzed data are derived from all cells with intact scatter.