AF647 Human/mouse/rat p44/42 MAPK Antibody [B5N16]

目录号:G4977

打印

生物描述

特异性
背景 p44/42 MAPK (Erk1/2) 是丝裂原活化蛋白激酶 (MAPK) 中一个广泛保守的亚群,它们是丝氨酸/苏氨酸蛋白激酶,参与重要的细胞过程,如增殖、分化、运动和细胞死亡。p44/42 MAPK (Erk1/2) 通路被多种胞外刺激激活,包括有丝分裂原、生长因子和细胞因子,使其成为癌症诊断和治疗的重要焦点。激活后,该信号通路启动一个三级蛋白激酶级联反应:一个 MAP 激酶激酶激酶 (MAPKKK 或 MAP3K),一个 MAP 激酶激酶 (MAPKK 或 MAP2K),最后是一个 MAP 激酶 (MAPK)。

使用信息

抗体应用 FCM, IHC, IF 推荐用量 0.2 μg ( 2.5 μL) / million cells in 100 µL volume
反应性 Human, Mouse, Rat, Hamster, Monkey, Mink, D. melanogaster, Zebrafish, Bovine, Dog, Pig, C. elegans, Chicken
抗体类型 Rabbit MW 42 kDa, 44 kDa
储存液配方 Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
储存条件
(自收到货起)
Store at 4°C in the dark to avoid freeze-thaw cycles

Application Data

FCM

Validated by Selleck

  • Fixation of Jurkat cells with 4% formaldehyde, after washing, cells were permeabilized by adding 1000 μL of pre-cooled 90% methanol, followed by washing, and then staining of cells using AF647 Human/mouse/rat p44/42 MAPK Antibody [B5N16] (Selleck Cat.: G4977) (green, with red blank control). Analyzed data are derived from all cells with intact scatter.