生物描述

特异性

ARF6 Rabbit Recombinant mAb detects endogenous levels of total ARF6.

背景

The ADP-ribosylation factors (Arfs) are a family of Ras-related, low molecular mass (∼20 kDa), GTP-binding proteins that are expressed in all eukaryotes. Like all GTPases, Arfs cycle between GDP-bound, inactive and GTP-bound, active states. In the active state, Arfs interact with proteins and other effector molecules to carry out their functions. Arf6 influences membrane trafficking and the actin cytoskeleton at the plasma membrane (PM). Arf6 activation and inactivation are catalyzed by guanine nucleotide exchange factors (GEFs) that facilitate GTP binding and GTPase-activating proteins (GAPs) that catalyze GTP hydrolysis. Arf6 changes the actin structure at the plasma membrane through activation of Rac, the Rho protein implicated in membrane ruffling. Arf6 is required for and enhances the ability of Rac to form plasma membrane ruffles. Arf6 can regulate dendritic branching in hippocampal neurons and neurite outgrowth in PC12 cells. It is also required in leukocytes for chemokine-stimulated migration across endothelial cells. In addition to its plasma membrane localization, Arf6 is associated with endosomal membranes in many cells. In total, Arf6 is involved in a wide variety of cellular events, including cell adhesion and migration, endocytosis, secretion, phagocytosis, and formation of invadopodia and is believed to accomplish these diverse functions by modulating plasma membrane rearrangement, actin cytoskeleton organization, and intracellular transport.

使用信息

抗体应用	WB, IF,ELISA,ELISA
稀释比例	WB IF 1:1000 1:50
反应性	Human Mouse Rat
MW (kDa)	20kDa
抗体类型
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Donaldson JG. J Biol Chem. 2003, 278(43):41573-6. [2] Klein S, et al. J Biol Chem. 2006, 281(18):12352-61.

Application Data

WB

IF