Atg4B Rabbit Recombinant mAb Datasheet

生物描述

特异性	Atg4B Rabbit Recombinant mAb detects endogenous level of Atg4B.
背景	Autophagy is an evolutionarily conserved catabolic pathway within all eukaryotic cells that plays a critical housekeeping function to remove senescent or damaged proteins and organelles through lysosome-mediated degradation. Autophagy also plays roles in generating substrates for maintaining cellular energy during times of nutrient insufficiency and hypoxia and in host defenses against intracellular pathogens. Atg4 plays dual roles in the progression of autophagy through the irreversible (proteolytic cleavage) and reversible (lipid deconjugation) modification of Atg8. ATG4B (autophagin-1) is an enzyme that is essential for autophagy in yeast and mammals. ATG4B (autophagin-1) has broad specificity for almost all of the mammalian Atg8 homologues and is the sole enzyme reported to efficiently cleave LC3 precursors and to deconjugate lipid from LC3-PE. Of the four autophagins identified (ATG4A, ATG4B, ATG4C, and ATG4D), ATG4B (autophagin-1) shows the highest catalytic efficiency for cleaving the C terminus of LC3B.

特异性

Atg4B Rabbit Recombinant mAb detects endogenous level of Atg4B.

背景

Autophagy is an evolutionarily conserved catabolic pathway within all eukaryotic cells that plays a critical housekeeping function to remove senescent or damaged proteins and organelles through lysosome-mediated degradation. Autophagy also plays roles in generating substrates for maintaining cellular energy during times of nutrient insufficiency and hypoxia and in host defenses against intracellular pathogens. Atg4 plays dual roles in the progression of autophagy through the irreversible (proteolytic cleavage) and reversible (lipid deconjugation) modification of Atg8. ATG4B (autophagin-1) is an enzyme that is essential for autophagy in yeast and mammals. ATG4B (autophagin-1) has broad specificity for almost all of the mammalian Atg8 homologues and is the sole enzyme reported to efficiently cleave LC3 precursors and to deconjugate lipid from LC3-PE. Of the four autophagins identified (ATG4A, ATG4B, ATG4C, and ATG4D), ATG4B (autophagin-1) shows the highest catalytic efficiency for cleaving the C terminus of LC3B.

使用信息

抗体应用

WB,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

44kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Yang Z, et al. J Biol Chem. 2015, 290(44):26549-61.

Application Data

WB

Validated by Selleck