Bad Rabbit Recombinant mAb

生物描述

特异性

Bad Rabbit Recombinant mAb detects endogenous level of Bad.

背景

The Bcl-2-associated death promoter (BAD) protein is a pro-apoptotic member of the Bcl-2 gene family which is involved in initiating apoptosis. BAD protein promote apoptosis through the intrinsic mitochondrial pathway. Bad can selectively bind Bcl-2, Bcl-XL and Bcl-w. Dephosphorylated BAD forms a heterodimer with Bcl-2 and Bcl-xL, inactivating them and thus allowing Bax/Bak-triggered apoptosis. When BAD is phosphorylated by Akt/protein kinase B (triggered by PIP3), it forms the BAD-(14-3-3)protein heterodimer. This leaves Bcl-2 free to inhibit Bax-triggered apoptosis. BAD phosphorylation is thus anti-apoptotic, and BAD dephosphorylation (e.g., by Ca2+-stimulated Calcineurin) is pro-apoptotic. The latter may be involved in neural diseases such as schizophrenia.

使用信息

抗体应用	WB, IHC,ELISA,ELISA
稀释比例	WB IHC 1:1000 1:50
反应性	Human Mouse Rat
MW (kDa)	23kDa
抗体类型	Rabbit
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Adachi M, et al. Cell Death Differ. 2002, 9(11):1240-7. [2] Brunelle JK, et al. J Cell Sci. 2009, 122(Pt 4):437-41.

Application Data

WB

IHC