Bax Rabbit Recombinant mAb Datasheet

生物描述

特异性	Bax Rabbit Recombinant mAb detects endogenous level of total Bax.
背景	Bax and Bak are two nuclear-encoded proteins present in higher eukaryotes that are able to pierce the mitochondrial outer membrane to mediate cell death by apoptosis. The mitochondrial pathway of apoptosis is regulated principally by the Bcl-2 protein family, whose members fall into three subclasses: the pro-apoptotic BH3-only proteins; the prosurvival Bcl-2-like proteins; and the pore-forming Bax and Bak proteins. Bax and Bak activity is regulated by other members of the Bcl-2 family. Bax translocation from the cytosol to the mitochondrial outer membrane is an important feature of apoptosis biology.

特异性

Bax Rabbit Recombinant mAb detects endogenous level of total Bax.

背景

Bax and Bak are two nuclear-encoded proteins present in higher eukaryotes that are able to pierce the mitochondrial outer membrane to mediate cell death by apoptosis. The mitochondrial pathway of apoptosis is regulated principally by the Bcl-2 protein family, whose members fall into three subclasses: the pro-apoptotic BH3-only proteins; the prosurvival Bcl-2-like proteins; and the pore-forming Bax and Bak proteins. Bax and Bak activity is regulated by other members of the Bcl-2 family. Bax translocation from the cytosol to the mitochondrial outer membrane is an important feature of apoptosis biology.

使用信息

抗体应用

WB, IHC, IF,ELISA,ELISA

稀释比例

WB	IHC	IF
1:1000	1:50	1:50

反应性

Human Mouse Rat Hamster

MW (kDa)

21kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Westphal D, et al. Biochim Biophys Acta. 2011, 1813(4):521-31.

Application Data

WB

Validated by Selleck

IHC

Validated by Selleck

IF

Validated by Selleck