Bim Rabbit Recombinant mAb

生物描述

特异性

Bim Rabbit Recombinant mAb detects endogenous levels of total Bim.

背景

Bcl-2 family proteins play a critical role in apoptosis by regulating permeabilization of the mitochondrial membrane. Proapoptotic BH3-only protein Bim plays an important role in Bax/Bak-mediated cytochrome c release and apoptosis. It binds to antiapoptotic proteins, and thus allow the proapoptotic multidomain proteins, Bax and Bak to form channels on the mitochondrial membrane leading to cytochrome c release and apoptosis. Normally, Bim is sequestered to the microtubule-associated dynein motor complex by binding to 8-kDa dynein light chain 8 (LC8 or DLC1), also called PIN. Bim is expressed in multiple lineages of cells including hematopoietic, epithelial, and neuronal cells. Phosphorylation of Bim at Ser69 by Erk1/2 and at Ser87 by Akt promotes cancer cell survival. Bim interacts with and stabilizes Mcl-1, which also contribute to cancer cell survival.

使用信息

抗体应用	WB, IHC,ELISA,ELISA
稀释比例	WB IHC 1:1000 1:50
反应性	Human Mouse Rat
MW (kDa)	22kDa
抗体类型	Rabbit
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Gogada R, et al. J Biol Chem. 2013, 288(1):368-81.

Application Data

WB

IHC