Biotin Human IFN-λ Antibody [4S.B3]

目录号:H0135

打印

生物描述

特异性
背景 IFNγ(干扰素γ,II型干扰素)是一种巨噬细胞活化因子和免疫干扰素,主要由T淋巴细胞和自然杀伤细胞在抗原、丝裂原、金黄色葡萄球菌肠毒素B、植物血凝素和其他细胞因子的刺激下产生。IFNγ是由两个146个氨基酸亚基组成的二聚体蛋白。IFNγ是一种糖蛋白,在功能上以约45 kDa的同二聚体形式存在。在SDS-PAGE上,由于不同的糖基化,IFNγ呈现为25、20和次要的15.5 kDa条带的组合。IFNγ同二聚体的生物活性具有高度的物种特异性。人类IFNγ与小鼠无交叉反应性。

使用信息

抗体应用 FCM 推荐用量 5 uL/million cells in 100 µL volume
反应性 Human, Mouse, Rat, Rhesus monkey
抗体类型 Mouse MW 17 kDa
储存液配方 Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
储存条件
(自收到货起)
Store at 4°C in the dark to avoid freeze-thaw cycles

Application Data

FCM

Validated by Selleck

  • Normal human peripheral blood leukocytes were stimulated with Cell Stimulation Cocktail for 5 hours. Subsequently, cells were treated with the Intracellular Fixation & Permeabilization Buffer Set, and stained using Biotin Human IFN-λ Antibody [4S.B3] (Product No.: H0135) and SA-PE, and CD4-FITC. Analyzed data are derived from total cells.