Biotin mouse CD275 Antibody [HK5.3]

目录号：G4920

打印

生物描述

特异性
背景 ICOSL（可诱导的T细胞共刺激配体）也称为CD275、B7RP-1和B7-H2。ICOSL是一种40 kDa的免疫检查点蛋白，属于Ig受体超家族。ICOSL结合后，ICOS信号共刺激T和B细胞应答。该配体在抗原呈递细胞上表达，包括脾脏B细胞、树突状细胞和巨噬细胞。ICOS信号传导也被认为对维持调节性T细胞稳态很重要。抗ICOSL抗体在体外和体内均阻断ICOSL与ICOS的结合，对小鼠的治疗会导致调节性T细胞的丧失。

使用信息

抗体应用 FCM 推荐用量 0.2 μg ( 0.4 μL) / million cells in 100 µL volume
反应性 Mouse
抗体类型 Rat MW 40 kDa
储存液配方 Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
储存条件
(自收到货起)		 Store at 4°C in the dark to avoid freeze-thaw cycles

Application Data

FCM

Validated by Selleck

  • Staining normal human peripheral blood mononuclear cells (HPBMCs) stimulated with 1μg/mL LPS for one day with biotin mouse CD275 Antibody (Clone: HK5.3) (Product Number: G4920), SA-AF647 and FITC CD45R Antibody (green, red is unstained control). Analysis was performed on total live cells.