Datasheet

生物描述

特异性	BTK Rabbit Recombinant mAb detects endogenous levels of total BTK.
背景	Bruton's agammaglobulinemia tyrosine kinase (Btk) is a cyto-plasmic tyrosine kinase important in B-lymphocyte development, differ-entiation, and signaling. Btk is a member of the Tec family of kinases. Activation of Btktriggers a cascade of signaling events that culminates in the generation ofcalcium mobilization and fluxes, cytoskeletal rearrangements, and tran-scriptional regulation involving nuclear factor-jB (NF-jB) and nuclearfactor of activated T cells (NFAT). Moreover, Btk activation is tightly regulated by a plethora of other signaling proteinsincluding protein kinase C (PKC), Sab⁄SH3BP5, and caveolin-1. It is predominantly expressed in B lymphocytes but not in plasma cells. Plasma membrane localization of Btk is a critical step in the phosphorylation and activation of Btk. Btk is continuouslyshuttling between the cytosol and the nucleus. As nuclear Btk is less phosphorylated as compared with the form residing in the cytosol, it is possible that a nuclear phosphataseis responsible and that this could be of functional importance for the regulation of this kinase.

特异性

BTK Rabbit Recombinant mAb detects endogenous levels of total BTK.

背景

Bruton's agammaglobulinemia tyrosine kinase (Btk) is a cyto-plasmic tyrosine kinase important in B-lymphocyte development, differ-entiation, and signaling. Btk is a member of the Tec family of kinases. Activation of Btktriggers a cascade of signaling events that culminates in the generation ofcalcium mobilization and fluxes, cytoskeletal rearrangements, and tran-scriptional regulation involving nuclear factor-jB (NF-jB) and nuclearfactor of activated T cells (NFAT). Moreover, Btk activation is tightly regulated by a plethora of other signaling proteinsincluding protein kinase C (PKC), Sab⁄SH3BP5, and caveolin-1. It is predominantly expressed in B lymphocytes but not in plasma cells. Plasma membrane localization of Btk is a critical step in the phosphorylation and activation of Btk. Btk is continuouslyshuttling between the cytosol and the nucleus. As nuclear Btk is less phosphorylated as compared with the form residing in the cytosol, it is possible that a nuclear phosphataseis responsible and that this could be of functional importance for the regulation of this kinase.

使用信息

抗体应用

wb,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human

MW (kDa)

76kDa

抗体类型

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Mohamed AJ, et al. Immunol Rev. 2009, 228(1):58-73.

Application Data

WB

Validated by Selleck