Calpain 2 Rabbit Recombinant mAb Datasheet

生物描述

特异性	Calpain 2 Rabbit Recombinant mAb detects endogenous level of total Calpain 2.
背景	Calpain is a cytoplasmic cysteine protease requiring calcium ions for activity. It is implicated in a variety of calcium-regulated cellular processes such as signal transduction, cell proliferation, cell cycle progression, differentiation, apoptosis, membrane fusion, and platelet activation while deregulation of its activity has been implicated in various pathological conditions such as neuronal degeneration, Alzheimer’s disease, metastasis, and cataract. Calpain exists in the cytosol as an inactive enzyme and translocates to membranes in response to increases in the cellular Ca2+ level. At the membrane, calpain is activated in the presence of Ca2+ and phospholipids. Activated calpain hydrolyzes substrate proteins at membranes or in cytosol after release from membranes. Calpain 2 is regarded as a ubiquitous calpain. It is a heterodimer composed of a large catalytic subunit of 80 kDa and a common regulatory subunit of 30 kDa (Calpain4) that dissociates upon activation.

特异性

Calpain 2 Rabbit Recombinant mAb detects endogenous level of total Calpain 2.

背景

Calpain is a cytoplasmic cysteine protease requiring calcium ions for activity. It is implicated in a variety of calcium-regulated cellular processes such as signal transduction, cell proliferation, cell cycle progression, differentiation, apoptosis, membrane fusion, and platelet activation while deregulation of its activity has been implicated in various pathological conditions such as neuronal degeneration, Alzheimer’s disease, metastasis, and cataract. Calpain exists in the cytosol as an inactive enzyme and translocates to membranes in response to increases in the cellular Ca2+ level. At the membrane, calpain is activated in the presence of Ca2+ and phospholipids. Activated calpain hydrolyzes substrate proteins at membranes or in cytosol after release from membranes. Calpain 2 is regarded as a ubiquitous calpain. It is a heterodimer composed of a large catalytic subunit of 80 kDa and a common regulatory subunit of 30 kDa (Calpain4) that dissociates upon activation.

使用信息

抗体应用

WB ,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

80kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck