Caspase-3 p12 Rabbit Recombinant mAb

生物描述

特异性

Caspase-3 p12 Rabbit Recombinant mAb detects endogenous level of total Caspase-3 p12.

背景

Caspase-3 is one of the main effector caspases in mammalian cells. Caspase-3 is synthesized as a dormant proenzyme (p32), consisting of a 3 kDa prodomain, the large subunit (p17) and the small subunit (p12). Maturation is executed via a two-step mechanism. First, the large and the small subunit are generated by proteolytic cleavage mediated via an initiator caspase or through pH-dependent autocatalytic processing. In a second step, the preactivated caspase-3 self-catalyses removal of its prodomain from the large subunit to generate the p17 polypeptide. The mature enzyme was shown to exist as a tetramer with two independent active sites, each containing elements from one large (p17) and one small (p12) subunit.

使用信息

抗体应用	WB, IHC, IF,ELISA,ELISA
稀释比例	WB IHC IF 1:1000 1:50 1:50
反应性	Human Mouse Rat
MW (kDa)	12,29,32kDa
抗体类型	Rabbit
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Tawa P, et al. Cell Death Differ. 2004, 11(4):439-47.

Application Data

WB

IHC

IF