Caspase-6 Rabbit Recombinant mAb Datasheet

生物描述

特异性	Caspase-6 Rabbit Recombinant mAb detects endogenous level of total Caspase-6.
背景	Caspases, a family of cysteine proteases, are major mediators of apoptosis and inflammation. Caspase-6 is classified as an apoptotic effector, and it mediates nuclear shrinkage during apoptosis, but it possesses unique activation and regulation mechanisms that differ from those of other effector caspases. Caspase-6, along with caspase-3 and caspase-7, is classified as an apoptotic effector. During apoptosis, caspase-6 localizes in the nucleus, cleaves the nuclear structural protein NuMA (nuclear mitotic apparatus protein) and the lamin proteins, and induces nuclear shrinkage and fragmentation. Furthermore, many transcription factors are substrates of caspase-6, including nuclear factor κB (NF-κB), special AT-rich sequence-binding protein 1 (SATB1), activating protein 2α (AP-2α), and CREB-binding protein (CBP). However, unlike the activity of caspase-3 and caspase-7, caspase-6 activity does not always induce apoptosis. Caspase-6 activity localizes to the cytoplasm and neurites of Alzheimer’s disease (AD) neurons without exhibiting the classical features of apoptosis. Caspase-6 is widely expressed in the brain and peripheral tissues. In addition to being involved in apoptosis, caspase-6 is highly involved in axon pruning during development and in adult brains.

特异性

Caspase-6 Rabbit Recombinant mAb detects endogenous level of total Caspase-6.

背景

Caspases, a family of cysteine proteases, are major mediators of apoptosis and inflammation. Caspase-6 is classified as an apoptotic effector, and it mediates nuclear shrinkage during apoptosis, but it possesses unique activation and regulation mechanisms that differ from those of other effector caspases. Caspase-6, along with caspase-3 and caspase-7, is classified as an apoptotic effector. During apoptosis, caspase-6 localizes in the nucleus, cleaves the nuclear structural protein NuMA (nuclear mitotic apparatus protein) and the lamin proteins, and induces nuclear shrinkage and fragmentation. Furthermore, many transcription factors are substrates of caspase-6, including nuclear factor κB (NF-κB), special AT-rich sequence-binding protein 1 (SATB1), activating protein 2α (AP-2α), and CREB-binding protein (CBP). However, unlike the activity of caspase-3 and caspase-7, caspase-6 activity does not always induce apoptosis. Caspase-6 activity localizes to the cytoplasm and neurites of Alzheimer’s disease (AD) neurons without exhibiting the classical features of apoptosis. Caspase-6 is widely expressed in the brain and peripheral tissues. In addition to being involved in apoptosis, caspase-6 is highly involved in axon pruning during development and in adult brains.

使用信息

抗体应用

WB, IF,ELISA,ELISA

稀释比例

WB	IF
1:1000	1:50

反应性

Human Mouse Rat

MW (kDa)

34kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Wang XJ, et al. Annu Rev Pharmacol Toxicol. 2015, 55:553-72.

Application Data

WB

Validated by Selleck

IF

Validated by Selleck