Datasheet

生物描述

特异性	CD276 Rabbit Recombinant mAb detects endogenous levels of total CD276.
背景	B7-H3 (CD276) is an important immune checkpoint member of the B7 and CD28 families. Induced on antigen presenting cells, CD276 plays an important role in the inhibition of T cell function. At the mRNA level, CD276 is ubiquitously found in non-lymphoid and lymphoid organs as liver, heart, prostate, spleen and thymus. Despite broad mRNA expression, protein expression is limited at steady state. CD276 is constitutively found on non-immune resting fibroblasts, endothelial cells (EC), osteoblasts, and amniotic fluid stem cells. Its expression is induced on immune cells, specifically antigen-presenting cells. The B7-H3 (CD276) pathway has a dual role in contributing to the regulation of innate immune responses: neuroblastoma cells are found to express B7-H3 on their cell surface, which protect them from NK cell-mediated lysis; CD276 also costimulates innate immunity by augmenting proinflammatory cytokines release from LPS-stimulated monocytes/macrophages, in both a Toll like receptor 4- and 2-dependent manner. Beyond the immune system, the B7-H3 pathway has a non-immunological role in promoting osteoblastic differentiation and bone mineralization in mice, ensuring normal bone formation.

特异性

CD276 Rabbit Recombinant mAb detects endogenous levels of total CD276.

背景

B7-H3 (CD276) is an important immune checkpoint member of the B7 and CD28 families. Induced on antigen presenting cells, CD276 plays an important role in the inhibition of T cell function. At the mRNA level, CD276 is ubiquitously found in non-lymphoid and lymphoid organs as liver, heart, prostate, spleen and thymus. Despite broad mRNA expression, protein expression is limited at steady state. CD276 is constitutively found on non-immune resting fibroblasts, endothelial cells (EC), osteoblasts, and amniotic fluid stem cells. Its expression is induced on immune cells, specifically antigen-presenting cells. The B7-H3 (CD276) pathway has a dual role in contributing to the regulation of innate immune responses: neuroblastoma cells are found to express B7-H3 on their cell surface, which protect them from NK cell-mediated lysis; CD276 also costimulates innate immunity by augmenting proinflammatory cytokines release from LPS-stimulated monocytes/macrophages, in both a Toll like receptor 4- and 2-dependent manner. Beyond the immune system, the B7-H3 pathway has a non-immunological role in promoting osteoblastic differentiation and bone mineralization in mice, ensuring normal bone formation.

使用信息

抗体应用

wb,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse

MW (kDa)

100kDa

抗体类型

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Elodie Picarda, et al. Clin Cancer Res. 2016, 22(14): 3425–3431.

Application Data

WB

Validated by Selleck