CD33 Rabbit Recombinant mAb Datasheet

生物描述

特异性	CD33 Rabbit Recombinant mAb detects endogenous level of CD33.
背景	CD33 is a myeloid specific member of the sialic acid-binding receptor family and is expressed highly on myeloid progenitor cells but at much lower levels in differentiated cells. Its expression is not seen in the earliest pluripotent progenitor cells, but it is expressed highly on cells committed to the myeloid lineage. CD33 expression is down-regulated with development of the myeloid lineage, resulting in low-level expression on peripheral granulocytes and tissue macrophages. Human CD33 has two tyrosine residues in its cytoplasmic domain (Y340 and Y358). When phosphorylated, these tyrosines could function as docking sites for the phosphatases, SHP-1 and/or SHP-2, enabling CD33 to function as an inhibitory receptor. CD33 belongs to a growing family of sialic acid-binding, immunoglobulin-like lectins (siglecs). It has two immunoglobulin-like domains and two cytoplasmic tyrosines. After phosphorylation, CD33 is capable of recruiting the protein tyrosine phosphatases SHP-1 and SHP-2, then negatively regulate cell activation.

特异性

CD33 Rabbit Recombinant mAb detects endogenous level of CD33.

背景

CD33 is a myeloid specific member of the sialic acid-binding receptor family and is expressed highly on myeloid progenitor cells but at much lower levels in differentiated cells. Its expression is not seen in the earliest pluripotent progenitor cells, but it is expressed highly on cells committed to the myeloid lineage. CD33 expression is down-regulated with development of the myeloid lineage, resulting in low-level expression on peripheral granulocytes and tissue macrophages. Human CD33 has two tyrosine residues in its cytoplasmic domain (Y340 and Y358). When phosphorylated, these tyrosines could function as docking sites for the phosphatases, SHP-1 and/or SHP-2, enabling CD33 to function as an inhibitory receptor. CD33 belongs to a growing family of sialic acid-binding, immunoglobulin-like lectins (siglecs). It has two immunoglobulin-like domains and two cytoplasmic tyrosines. After phosphorylation, CD33 is capable of recruiting the protein tyrosine phosphatases SHP-1 and SHP-2, then negatively regulate cell activation.

使用信息

抗体应用

WB,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human

MW (kDa)

65-75kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Paul SP, et al. Blood. 2000, 96(2):483-90.

Application Data

WB

Validated by Selleck