Datasheet

生物描述

特异性	CD47 Rabbit Recombinant mAb detects endogenous levels of total CD47.
背景	Cluster of differentiation 47 (CD47) (also known as integrin-associated protein) is a ubiquitously expressed glycoprotein of the immunoglobulin superfamily that plays a critical role in self-recognition. It is a membrane protein involved in β3 integrin-mediated signaling on leukocytes and also interacts with thrombospondin-1, signal regulatory protein-alpha (SIRPα), and others to regulate various cellular functions including cell migration, axon extension, cytokine production, and T cell activation. CD47 inhibit phagocytosis of erythrocytes by macrophages of the reticuloendothelial system. CD47 exerts its inhibitory effect through binding to SIRPα on the macrophage, which induces inhibitory signaling by the immunoreceptor tyrosine-based inhibition motifs (ITIMs) residing in the cytoplasmic tail of SIRPα. Many solid and hematologic malignancies express CD47 on their cell surface to display an anti-phagocytic signal to SIRPα-expressing myeloid cells and evade destruction by innate and adaptive immune system. Administration of anti-CD47 blocking antibodies has been enormously successful in various pre-clinical models.

特异性

CD47 Rabbit Recombinant mAb detects endogenous levels of total CD47.

背景

Cluster of differentiation 47 (CD47) (also known as integrin-associated protein) is a ubiquitously expressed glycoprotein of the immunoglobulin superfamily that plays a critical role in self-recognition. It is a membrane protein involved in β3 integrin-mediated signaling on leukocytes and also interacts with thrombospondin-1, signal regulatory protein-alpha (SIRPα), and others to regulate various cellular functions including cell migration, axon extension, cytokine production, and T cell activation. CD47 inhibit phagocytosis of erythrocytes by macrophages of the reticuloendothelial system. CD47 exerts its inhibitory effect through binding to SIRPα on the macrophage, which induces inhibitory signaling by the immunoreceptor tyrosine-based inhibition motifs (ITIMs) residing in the cytoplasmic tail of SIRPα. Many solid and hematologic malignancies express CD47 on their cell surface to display an anti-phagocytic signal to SIRPα-expressing myeloid cells and evade destruction by innate and adaptive immune system. Administration of anti-CD47 blocking antibodies has been enormously successful in various pre-clinical models.

使用信息

抗体应用

wb,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

55kDa

抗体类型

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck