CD86 Rabbit Recombinant mAb Datasheet

生物描述

特异性	CD86 Rabbit Recombinant mAb detects endogenous level of total CD86.
背景	CD86 is a costimulatory molecule that is mainly expressed on APCs such as dendritic cells (DCs). CD86 on DCs engages with CD28 on T cells, which provides T cells with the costimulatory signals that significantly lower the activation threshold and allow naive T cells to be readily activated. DCs dynamically regulate CD86 levels depending on their need to harness or reduce their T cell-activating ability. DCs upregulate CD86 expression following their contact with microbial Ags, which presumably facilitates activation of microbe-specific T cells capable of combating infection. DCs downregulate CD86 expression following exposure to IL-10, an immune-suppressive cytokine produced by various immune cells. CD86 is ubiquitinated in DCs and that this ubiquitination is also mediated by MARCH1. The ubiquitination of CD86 plays an important role in DCs to regulate their CD86 expression and their T cell-activating abilities both at rest and in response to autocrine IL-10.

特异性

CD86 Rabbit Recombinant mAb detects endogenous level of total CD86.

背景

CD86 is a costimulatory molecule that is mainly expressed on APCs such as dendritic cells (DCs). CD86 on DCs engages with CD28 on T cells, which provides T cells with the costimulatory signals that significantly lower the activation threshold and allow naive T cells to be readily activated. DCs dynamically regulate CD86 levels depending on their need to harness or reduce their T cell-activating ability. DCs upregulate CD86 expression following their contact with microbial Ags, which presumably facilitates activation of microbe-specific T cells capable of combating infection. DCs downregulate CD86 expression following exposure to IL-10, an immune-suppressive cytokine produced by various immune cells. CD86 is ubiquitinated in DCs and that this ubiquitination is also mediated by MARCH1. The ubiquitination of CD86 plays an important role in DCs to regulate their CD86 expression and their T cell-activating abilities both at rest and in response to autocrine IL-10.

使用信息

抗体应用

WB, IHC,ELISA,ELISA

稀释比例

WB	IHC
1:1000	1:50

反应性

Human Rat

MW (kDa)

70kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Baravalle G, et al. J Immunol. 2011, 187(6):2966-73.

Application Data

WB

Validated by Selleck

IHC

Validated by Selleck