Cdc25B Rabbit Recombinant mAb Datasheet

生物描述

特异性	Cdc25B Rabbit Recombinant mAb detects endogenous level of total Cdc25B.
背景	Cdc25 phosphatases play key roles in cell cycle progression by activating cyclin-dependent kinases. In human cells, cdc25 proteins are encoded by a multigene family, consisting of cdc25A, cdc25B and cdc25C. While cdc25A plays a crucial role at the G1/S phase transition, cdc25C is involved in the dephosphorylation and activation of the mitotic kinase, cdc2/cyclinB. Cdc25B is a mitotic regulator that might act as a ‘starter phosphatase’ to initiate the positive feedback loop at the entry into M phase. Cdc25B is an unstable protein that might be important for the G2/M phase transition. cdc25B phosphatase is activated during late S phase/early G2 phase prior to the activation of cdc25C and shows a high substrate specificity for cdc2/cyclinA during S-phase while during G2-phase the activity increases towards cdc2/cyclinB as substrate.

特异性

Cdc25B Rabbit Recombinant mAb detects endogenous level of total Cdc25B.

背景

Cdc25 phosphatases play key roles in cell cycle progression by activating cyclin-dependent kinases. In human cells, cdc25 proteins are encoded by a multigene family, consisting of cdc25A, cdc25B and cdc25C. While cdc25A plays a crucial role at the G1/S phase transition, cdc25C is involved in the dephosphorylation and activation of the mitotic kinase, cdc2/cyclinB. Cdc25B is a mitotic regulator that might act as a ‘starter phosphatase’ to initiate the positive feedback loop at the entry into M phase. Cdc25B is an unstable protein that might be important for the G2/M phase transition. cdc25B phosphatase is activated during late S phase/early G2 phase prior to the activation of cdc25C and shows a high substrate specificity for cdc2/cyclinA during S-phase while during G2-phase the activity increases towards cdc2/cyclinB as substrate.

使用信息

抗体应用

WB ,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human

MW (kDa)

64kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Lammer C, et al. J Cell Sci. 1998, 111 ( Pt 16):2445-53.

Application Data

WB

Validated by Selleck