Cdc25C Rabbit Recombinant mAb Datasheet

生物描述

特异性	Cdc25C Rabbit Recombinant mAb detects endogenous level of total Cdc25C.
背景	CDC25 phosphatases, which are believed to be important regulators of cell cycle progression, dominate entry into mitosis by regulating the activation of CDK1/cyclin B. Catalyzed by these dual specificity phosphatases, cyclin/CDKs are dephosphorylated and actived after removal of inhibitory phosphate groups from Thr14 and Tyr15. In higher-eukaryotic cells, three isoforms of CDC25 denoted CDC25A, CDC25B and CDC25C exist. While Cdc25A is involved in the regulation of the Gl-to-S phase transition, Cdc25B and Cdc25C play an important role at the G2-to-M transition. Cdc25C interacts with two isoforms of 14-3-3, namely 14-3-3ε and 14-3-3ζ. The binding of 14-3-3 proteins to Cdc25 is high during interphase and absent during mitosis.

特异性

Cdc25C Rabbit Recombinant mAb detects endogenous level of total Cdc25C.

背景

CDC25 phosphatases, which are believed to be important regulators of cell cycle progression, dominate entry into mitosis by regulating the activation of CDK1/cyclin B. Catalyzed by these dual specificity phosphatases, cyclin/CDKs are dephosphorylated and actived after removal of inhibitory phosphate groups from Thr14 and Tyr15. In higher-eukaryotic cells, three isoforms of CDC25 denoted CDC25A, CDC25B and CDC25C exist. While Cdc25A is involved in the regulation of the Gl-to-S phase transition, Cdc25B and Cdc25C play an important role at the G2-to-M transition. Cdc25C interacts with two isoforms of 14-3-3, namely 14-3-3ε and 14-3-3ζ. The binding of 14-3-3 proteins to Cdc25 is high during interphase and absent during mitosis.

使用信息

抗体应用

WB, IF,ELISA,ELISA

稀释比例

WB	IF
1:1000	1:50

反应性

Human

MW (kDa)

60kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck

IF

Validated by Selleck