CDC5 Rabbit Recombinant mAb Datasheet

生物描述

特异性	CDC5 Rabbit Recombinant mAb detects endogenous level of total CDC5.
背景	CDC5 is a MYB-related protein that exists in plants, animals, and fungi. CDC5 is considered a putative transcription factor, as it is a MYB (a transcription factor)-related protein. In human and yeast, CDC5 has been shown to act as a component of spliceosome to participate in mRNA splicing. In Arabidopsis, CDC5 binds DNA and is required for development and immunity to bacteria infection. Cdc5 has evolved into a master regulator of the unique meiosis I chromosome segregation pattern. Although CDC5 activity is not needed during meiosis II, Cdc5 is likely to affect meiosis II chromosome segregation by functions it executes during meiosis I. Cdc5 sets itself apart from most other cell cycle kinases through its ability to phosphorylate different substrates at different cell-cycle stages. Several Cdc5 substrates, Rec8 S136 and S179, Lrs4, and Clb1, are phosphorylated in meiosis I but significantly less so in meiosis II. CDC5 Suppresses the DNA Damage Checkpoint and can bind and phosphorylate Rad53. Cdc5 phosphorylation might affect the positioning of Rad53 with respect to either other Rad53 molecules or Rad9 so as to prevent proper Rad53 trans-autophosphorylation.

特异性

CDC5 Rabbit Recombinant mAb detects endogenous level of total CDC5.

背景

CDC5 is a MYB-related protein that exists in plants, animals, and fungi. CDC5 is considered a putative transcription factor, as it is a MYB (a transcription factor)-related protein. In human and yeast, CDC5 has been shown to act as a component of spliceosome to participate in mRNA splicing. In Arabidopsis, CDC5 binds DNA and is required for development and immunity to bacteria infection. Cdc5 has evolved into a master regulator of the unique meiosis I chromosome segregation pattern. Although CDC5 activity is not needed during meiosis II, Cdc5 is likely to affect meiosis II chromosome segregation by functions it executes during meiosis I. Cdc5 sets itself apart from most other cell cycle kinases through its ability to phosphorylate different substrates at different cell-cycle stages. Several Cdc5 substrates, Rec8 S136 and S179, Lrs4, and Clb1, are phosphorylated in meiosis I but significantly less so in meiosis II. CDC5 Suppresses the DNA Damage Checkpoint and can bind and phosphorylate Rad53. Cdc5 phosphorylation might affect the positioning of Rad53 with respect to either other Rad53 molecules or Rad9 so as to prevent proper Rad53 trans-autophosphorylation.

使用信息

抗体应用

WB, IF,ELISA,ELISA

稀释比例

WB	IF
1:1000	1:50

反应性

Human Mouse Rat

MW (kDa)

100kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck

IF

Validated by Selleck