Citrate synthetase Rabbit Recombinant mAb Datasheet

生物描述

特异性	Citrate synthetase Rabbit Recombinant mAb detects endogenous level of total Citrate synthetase.
背景	Citrate synthase is a component of nearly all living cells and plays a key role in the central metabolic pathway of aerobic organisms, the citric acid cycle. The citric acid cycle functions as a source of two reducing equivalents for the electron transport chain and as a source of intermediates required for the biosynthesis of amino acids for ketogenesis, lipogenesis, and gluconeogenesis. Citrate synthase catalyzes an important step within the cycle, the Claisen condensation of acetyl-Coenzyme A with oxaloacetate to form citrate; and it is the only enzyme in the cycle that can catalyze the formation of a carbon-carbon bond. Citrate acts not only as a substrate for the citric acid cycle within the mitochondria, but also as an acetyl donor for acetyl-Coenzyme A synthesis by ATP-citrate-Iyase outside the mitochondria after transport through the mitochondrial membrane.

特异性

Citrate synthetase Rabbit Recombinant mAb detects endogenous level of total Citrate synthetase.

背景

Citrate synthase is a component of nearly all living cells and plays a key role in the central metabolic pathway of aerobic organisms, the citric acid cycle. The citric acid cycle functions as a source of two reducing equivalents for the electron transport chain and as a source of intermediates required for the biosynthesis of amino acids for ketogenesis, lipogenesis, and gluconeogenesis. Citrate synthase catalyzes an important step within the cycle, the Claisen condensation of acetyl-Coenzyme A with oxaloacetate to form citrate; and it is the only enzyme in the cycle that can catalyze the formation of a carbon-carbon bond. Citrate acts not only as a substrate for the citric acid cycle within the mitochondria, but also as an acetyl donor for acetyl-Coenzyme A synthesis by ATP-citrate-Iyase outside the mitochondria after transport through the mitochondrial membrane.

使用信息

抗体应用

WB,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

45kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Wiegand G, et al. Annu Rev Biophys Biophys Chem. 1986, 15:97-117.

Application Data

WB

Validated by Selleck