Datasheet

生物描述

特异性	CNPase Rabbit Recombinant mAb detects endogenous levels of total CNPase.
背景	CNPase is a protein that hydrolyzes cyclic nucleotides to monophosphates (2' nucleotides). CNPase has two isoforms, CNPase1 (46 kDa) and CNPase2 (48 kDa), which are encoded separately by different promoters of the same gene and have different structural properties and subcellular localizations. A considerable level of CNPase expression and CNPase-like enzyme activity were found in membranes of cells in the spleen, liver, kidney, heart, and skeletal muscles. CNPase expression and its enzyme activity is localized in mitochondria, specifically in the mitochondrial membranes. CNPase is present in diverse eukaryotes and it is reported to be expressed at low levels in many mammalian tissues, at higher levels in dendritic cells, and at extremely high levels in oligodendrocytes, the major glial cells in the human brain. CNPase may be implicated in the regulation of mitochondrial Ca2+ fluxes and may regulate the mitochondrial permeability transition pore.

特异性

CNPase Rabbit Recombinant mAb detects endogenous levels of total CNPase.

背景

CNPase is a protein that hydrolyzes cyclic nucleotides to monophosphates (2' nucleotides). CNPase has two isoforms, CNPase1 (46 kDa) and CNPase2 (48 kDa), which are encoded separately by different promoters of the same gene and have different structural properties and subcellular localizations. A considerable level of CNPase expression and CNPase-like enzyme activity were found in membranes of cells in the spleen, liver, kidney, heart, and skeletal muscles. CNPase expression and its enzyme activity is localized in mitochondria, specifically in the mitochondrial membranes. CNPase is present in diverse eukaryotes and it is reported to be expressed at low levels in many mammalian tissues, at higher levels in dendritic cells, and at extremely high levels in oligodendrocytes, the major glial cells in the human brain. CNPase may be implicated in the regulation of mitochondrial Ca2+ fluxes and may regulate the mitochondrial permeability transition pore.

使用信息

抗体应用

wb,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

48kDa

抗体类型

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Yang L, et al. J Neuroinflammation. 2014, 11:148.

Application Data

WB

Validated by Selleck