Cortactin Rabbit Recombinant mAb Datasheet

生物描述

特异性	Cortactin Rabbit Recombinant mAb detects endogenous level of total Cortactin.
背景	Cortactin is an actin-nucleation-promoting factor that is shown to colocalize with cortical F-actin at the cell periphery. It acts downstream of E-cadherin activated by Src kinase and is required for actin polymerization at these cell-cell adhesions. Cortactin can activate the Arp2/3 complex and stabilize branched actin networks. In addition to interactions with its numerous binding partners, cortactin activity is also regulated by several post-translational modifications. Cortactin is phosphorylated at tyrosine residues in response to signaling downstream of different upstream receptors, which include integrin- and cadherin-adhesion receptors and growth factor receptors. Several nonreceptor tyrosine kinases have been implicated in the phosphorylation of cortactin, including Src family kinases, ABL family kinases, FER, and Syk. Cortactin is also phosphorylated by several serine/threonine kinases. It integrates signals from diverse upstream signaling cascades. In addition to phosphorylation, cortactin is also regulated through acetylation by histone acetyltransferase p300, and deacetylation by histone deacetylase 6 (HDAC6) and sirtuin-1 (SIRT1). Acetylation of cortactin regulates binding of F-actin. Cortactin is overexpressed in many cancers and is crucial for cancer cell invasion and metastasis. It is essential for the formation and function of invadopodia.

特异性

Cortactin Rabbit Recombinant mAb detects endogenous level of total Cortactin.

背景

Cortactin is an actin-nucleation-promoting factor that is shown to colocalize with cortical F-actin at the cell periphery. It acts downstream of E-cadherin activated by Src kinase and is required for actin polymerization at these cell-cell adhesions. Cortactin can activate the Arp2/3 complex and stabilize branched actin networks. In addition to interactions with its numerous binding partners, cortactin activity is also regulated by several post-translational modifications. Cortactin is phosphorylated at tyrosine residues in response to signaling downstream of different upstream receptors, which include integrin- and cadherin-adhesion receptors and growth factor receptors. Several nonreceptor tyrosine kinases have been implicated in the phosphorylation of cortactin, including Src family kinases, ABL family kinases, FER, and Syk. Cortactin is also phosphorylated by several serine/threonine kinases. It integrates signals from diverse upstream signaling cascades. In addition to phosphorylation, cortactin is also regulated through acetylation by histone acetyltransferase p300, and deacetylation by histone deacetylase 6 (HDAC6) and sirtuin-1 (SIRT1). Acetylation of cortactin regulates binding of F-actin. Cortactin is overexpressed in many cancers and is crucial for cancer cell invasion and metastasis. It is essential for the formation and function of invadopodia.

使用信息

抗体应用

WB,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

80kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] MacGrath SM, et al. J Cell Sci. 2012, 125(Pt 7):1621-6.

Application Data

WB

Validated by Selleck