Desmin Rabbit Recombinant mAb Datasheet

生物描述

特异性	Desmin Rabbit Recombinant mAb detects endogenous level of total desmin.
背景	Desmin is a muscle-specific protein and a key subunit of the intermediate filament in cardiac, skeletal and smooth muscles. Desmin filaments are mainly located at the periphery of Z-disk of striated muscles and at the dense bodies of smooth muscle cells. It plays an essential role in maintaining muscle cytoarchitecture by forming a three-dimensional scaffold around the myofibrillar Z-disk and by connecting the entire contractile apparatus to the subsarcolemmal cytoskeleton, the nuclei, and other organelles. Desmin also forms longitudinal connections between the peripheries of successive Z-disks and along the plasma membranes of striated muscle cells. It is particularly abundant at the myotendinous junctions and at the neuromuscular junctions of skeletal muscle, and in the intercalated disks, the attachment between cardiomyocytes in cardiac muscle. Desmin may be extremely important for the positioning, distribution, and function of mitochondria. Desmin filaments are involved in the generation of active and passive forces, possibly by supporting sarcomere alignment or force transmission. The protein is also implicated in tissue repair and regeneration. It is important in cell-matrix interactions, acting via its anchorage points on dense bodies, membrane-associated dense bands, and costameres at the sarcolemma.

特异性

Desmin Rabbit Recombinant mAb detects endogenous level of total desmin.

背景

Desmin is a muscle-specific protein and a key subunit of the intermediate filament in cardiac, skeletal and smooth muscles. Desmin filaments are mainly located at the periphery of Z-disk of striated muscles and at the dense bodies of smooth muscle cells. It plays an essential role in maintaining muscle cytoarchitecture by forming a three-dimensional scaffold around the myofibrillar Z-disk and by connecting the entire contractile apparatus to the subsarcolemmal cytoskeleton, the nuclei, and other organelles. Desmin also forms longitudinal connections between the peripheries of successive Z-disks and along the plasma membranes of striated muscle cells. It is particularly abundant at the myotendinous junctions and at the neuromuscular junctions of skeletal muscle, and in the intercalated disks, the attachment between cardiomyocytes in cardiac muscle. Desmin may be extremely important for the positioning, distribution, and function of mitochondria. Desmin filaments are involved in the generation of active and passive forces, possibly by supporting sarcomere alignment or force transmission. The protein is also implicated in tissue repair and regeneration. It is important in cell-matrix interactions, acting via its anchorage points on dense bodies, membrane-associated dense bands, and costameres at the sarcolemma.

使用信息

抗体应用

WB, IHC,ELISA,ELISA

稀释比例

WB	IHC
1:1000	1:50

反应性

Human Mouse Rat

MW (kDa)

53kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Paulin D, et al. Exp Cell Res. 2004, 301(1):1-7.

Application Data

WB

Validated by Selleck

IHC

Validated by Selleck