DHFR Rabbit Recombinant mAb Datasheet

生物描述

特异性	DHFR Rabbit Recombinant mAb detects endogenous level of total DHFR.
背景	Dihydrofolate reductase, or DHFR, is a ubiquitous enzyme found in all organisms. The enzyme catalyzes the reduction of 7,8-dihydrofolate (DHF) to 5,6,7,8-tetrahydrofolate (THF) by stereospecific hydride transfer from the NADPH cofactor to the C6 atom of the pterin ring with concomitant protonation at N5. DHFR plays a central role in maintenance of cellular pools of THF and its derivatives, which are essential for purine and thymidylate synthesis and hence for cell growth and proliferation.

特异性

DHFR Rabbit Recombinant mAb detects endogenous level of total DHFR.

背景

Dihydrofolate reductase, or DHFR, is a ubiquitous enzyme found in all organisms. The enzyme catalyzes the reduction of 7,8-dihydrofolate (DHF) to 5,6,7,8-tetrahydrofolate (THF) by stereospecific hydride transfer from the NADPH cofactor to the C6 atom of the pterin ring with concomitant protonation at N5. DHFR plays a central role in maintenance of cellular pools of THF and its derivatives, which are essential for purine and thymidylate synthesis and hence for cell growth and proliferation.

使用信息

抗体应用

WB, IHC,ELISA,ELISA

稀释比例

WB	IHC
1:1000	1:50

反应性

Human Mouse Rat

MW (kDa)

21kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Schnell JR, et al. Annu Rev Biophys Biomol Struct. 2004, 33:119-40.

Application Data

WB

Validated by Selleck

IHC

Validated by Selleck