生物描述

特异性

E1 Ubiquitin Activating Enzyme Rabbit Recombinant mAb detects endogenous levels of total E1 Ubiquitin Activating Enzyme

背景

The ubiquitin-activating enzyme UBA1 (E1) uses ATP to adenylate and then bind a ubiquitin molecule. Subsequently, a second ubiquitin molecule is then adenylated and bound to a different site of the same E1 enzyme. The E1 enzyme then transfers a ubiquitin molecule to the ubiquitin-conjugating enzyme E2. In the final step, the E2 enzyme transfers the ubiquitin to the target protein with the help of the ubiquitin ligase E3, resulting in ubiquitination of the target proteins with chains of 4 or more ubiquitins linked through Lysine-48 (K48) of ubiquitin. K48-polyubiquitinated proteins are then recognized, unfolded, and degraded by the proteasome enzyme complex. Through this pathway, the cell rids itself of excess and misfolded proteins and regulates biologic processes, including cellular proliferation.

使用信息

抗体应用	WB, IHC,ELISA,ELISA
稀释比例	WB IHC 1:1000 1:50
反应性	Human Mouse Rat
MW (kDa)	118kDa
抗体类型
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Xu GW, et al. Blood. 2010, 115(11):2251-9.

Application Data

WB

IHC