EBP50 Rabbit Recombinant mAb Datasheet

生物描述

特异性	EBP50 Rabbit Recombinant mAb detects endogenous level of total EBP50.
背景	EBP50 (also known as NHERF1), a 55-kDa phosphoprotein, was first identified as a cofactor essential for protein kinase A-mediated inhibition of Na+/H+ exchanger isoform 3 (NHE3). EBP50 contains two PDZ domains (PDZ1 and PDZ2) implicated in multiple protein-protein interactions, and an ERM domain, which binds to the actin-associated ERM proteins (ezrin, radixin, moesin, and merlin). EBP50 was also found to interact with a small number of transmembrane proteins such as the cystic fibrosis transmembrane conductance regulator (CFTR), the P2Y1 purinergic receptor, the platelet-derived growth factor receptor, the β2-adrenergic receptor, the B1 subunit of the H+-ATPase, and the type IIa sodium phosphate cotransporter. EBP50 also associates with the phospholipases C (PLC)-β1/β2, and with the TRP4 and TRP5 calcium channels to form a PLC-β1/2-TRP4/5-EBP50 protein complex. The EBP50 protein can also bind through its PDZ domains to various intracellular proteins, including GRK6A, EPI64, and Yes-associated protein 65.

特异性

EBP50 Rabbit Recombinant mAb detects endogenous level of total EBP50.

背景

EBP50 (also known as NHERF1), a 55-kDa phosphoprotein, was first identified as a cofactor essential for protein kinase A-mediated inhibition of Na+/H+ exchanger isoform 3 (NHE3). EBP50 contains two PDZ domains (PDZ1 and PDZ2) implicated in multiple protein-protein interactions, and an ERM domain, which binds to the actin-associated ERM proteins (ezrin, radixin, moesin, and merlin). EBP50 was also found to interact with a small number of transmembrane proteins such as the cystic fibrosis transmembrane conductance regulator (CFTR), the P2Y1 purinergic receptor, the platelet-derived growth factor receptor, the β2-adrenergic receptor, the B1 subunit of the H+-ATPase, and the type IIa sodium phosphate cotransporter. EBP50 also associates with the phospholipases C (PLC)-β1/β2, and with the TRP4 and TRP5 calcium channels to form a PLC-β1/2-TRP4/5-EBP50 protein complex. The EBP50 protein can also bind through its PDZ domains to various intracellular proteins, including GRK6A, EPI64, and Yes-associated protein 65.

使用信息

抗体应用

WB, IHC,ELISA,ELISA

稀释比例

WB	IHC
1:1000	1:50

反应性

Human Rat

MW (kDa)

50kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Moulay Driss Rochdi, et al. JBC. 2002, 277: 40751-40759.

Application Data

WB

Validated by Selleck

IHC

Validated by Selleck