Datasheet

生物描述

特异性	Endothelin B Receptor Rabbit Recombinant mAb detects endogenous levels of total Endothelin B Receptor.
背景	Endothelin B Receptor (ETBR) is a G-protein coupled receptor. ETBR differs from ETAR in its binding affinity to ET-1, and this may ultimately affect the fate of the receptor and ET-1. Both ETAR and ETBR are rapidly internalized upon agonist stimulation, a process that involves GRK, arrestin, dynamin and clathrin. Internalized ETAR and ETBR are directed to Rab5 positive early endosomes (sorting endosomes), then targeted to different intracellular fates. ETAR is directed to the pericentriolar compartment for recycling, and subsequently reappears back at the plasma membrane in a ligand-unbound state. Ligand-bound ETBR is internalized, but, unlike ETAR, it is sorted to the late endosomes/lysosomes for degradation--a major pathway for ET-1 "clearance". ETBR predominates in endothelial cells and is present in low densities in VSMCs of some vascular beds such as the aorta, coronary arteries, mesenteric arteries and veins of experimental animals and in human mammary artery. It is also expressed in vascular adventitial fibroblasts, the myocardium, renal tubules, airway smooth muscle, hepatocytes, osteoblasts, central and peripheral neurons, endocrine tissues and reproductive tract.

特异性

Endothelin B Receptor Rabbit Recombinant mAb detects endogenous levels of total Endothelin B Receptor.

背景

Endothelin B Receptor (ETBR) is a G-protein coupled receptor. ETBR differs from ETAR in its binding affinity to ET-1, and this may ultimately affect the fate of the receptor and ET-1. Both ETAR and ETBR are rapidly internalized upon agonist stimulation, a process that involves GRK, arrestin, dynamin and clathrin. Internalized ETAR and ETBR are directed to Rab5 positive early endosomes (sorting endosomes), then targeted to different intracellular fates. ETAR is directed to the pericentriolar compartment for recycling, and subsequently reappears back at the plasma membrane in a ligand-unbound state. Ligand-bound ETBR is internalized, but, unlike ETAR, it is sorted to the late endosomes/lysosomes for degradation--a major pathway for ET-1 "clearance". ETBR predominates in endothelial cells and is present in low densities in VSMCs of some vascular beds such as the aorta, coronary arteries, mesenteric arteries and veins of experimental animals and in human mammary artery. It is also expressed in vascular adventitial fibroblasts, the myocardium, renal tubules, airway smooth muscle, hepatocytes, osteoblasts, central and peripheral neurons, endocrine tissues and reproductive tract.

使用信息

抗体应用

wb,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human

MW (kDa)

50kDa

抗体类型

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Mazzuca MQ, et al. Biochem Pharmacol. 2012, 84(2):147-62.

Application Data

WB

Validated by Selleck