生物描述

特异性

FEN1 Rabbit Recombinant mAb detects endogenous levels of total FEN1.

背景

Flap endonuclease-1 (FEN1) is a multifunctional, structure-specific nuclease that has a critical role in maintaining human genome stability. FEN1 has 5' flap endonuclease (FEN), 5'–3' exonuclease (EXO) and gap endonuclease (GEN) activities. The main function of FEN1 in replication is proposed to be the removal of displaced RNA-DNA primers synthesized by DNA polymerase α-primase during discontinuous lagging-strand replication. Consistent with its crucial role in DNA replication, human FEN1 is highly expressed in all proliferative tissues. FEN1 is also implicated in mitochondrial genome maintenance.

使用信息

抗体应用	WB, IHC, IF,ELISA,ELISA
稀释比例	WB IHC IF 1:1000 1:50 1:50
反应性	Human Mouse Rat
MW (kDa)	42kDa
抗体类型
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Tsutakawa SE, et al. Cell. 2011, 145(2):198-211. [2] Larsen E, et al. Mol Cell Biol. 2003, 23(15):5346-53.

Application Data

WB

IHC

IF