Datasheet

生物描述

特异性	Fibulin 5 Rabbit Recombinant mAb detects endogenous levels of total Fibulin 5.
背景	Fibulin-5 is a glycoprotein secreted by various cell types, including vascular smooth muscle cells (SMCs), fibroblasts, and endothelial cells. It is an integrin-binding member of the Class II fibulin subfamily which also include fibulin-3, fibulin-4 and fibulin-7. Fibulin-5 contributes to the formation of elastic fibers by binding to structural components including tropoelastin and fibrillin-1, and to cross-linking enzymes, aiding elastic fiber assembly. Fibulin-5 enhances substrate attachment of endothelial cells, while inhibiting migration and proliferation in a cell type- and context-dependent manner. It may block angiogenesis by inducing the anti-angiogenic molecule thrompospondin-1, by antagonizing VEGF165-mediated signaling, and/or by antagonizing fibronectin-mediated signaling through directly binding and blocking the α5β1 fibronectin receptor. Fibulin-5 is shown to be strongly expressed in the embryonic vasculature and neural crest, but is down-regulated in all adult tissues, except in the uterus where active remodeling and angiogenesis takes place. It is reactivated in injured vessels, including the neointima induced by balloon withdrawal injury, and in atherosclerotic plaques in the mouse model of hypercholesterolemia.

特异性

Fibulin 5 Rabbit Recombinant mAb detects endogenous levels of total Fibulin 5.

背景

Fibulin-5 is a glycoprotein secreted by various cell types, including vascular smooth muscle cells (SMCs), fibroblasts, and endothelial cells. It is an integrin-binding member of the Class II fibulin subfamily which also include fibulin-3, fibulin-4 and fibulin-7. Fibulin-5 contributes to the formation of elastic fibers by binding to structural components including tropoelastin and fibrillin-1, and to cross-linking enzymes, aiding elastic fiber assembly. Fibulin-5 enhances substrate attachment of endothelial cells, while inhibiting migration and proliferation in a cell type- and context-dependent manner. It may block angiogenesis by inducing the anti-angiogenic molecule thrompospondin-1, by antagonizing VEGF165-mediated signaling, and/or by antagonizing fibronectin-mediated signaling through directly binding and blocking the α5β1 fibronectin receptor. Fibulin-5 is shown to be strongly expressed in the embryonic vasculature and neural crest, but is down-regulated in all adult tissues, except in the uterus where active remodeling and angiogenesis takes place. It is reactivated in injured vessels, including the neointima induced by balloon withdrawal injury, and in atherosclerotic plaques in the mouse model of hypercholesterolemia.

使用信息

抗体应用

wb,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse

MW (kDa)

50kDa

抗体类型

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Hiromi Yanagisawa, et al. J Cell Commun Signal. 2009, 3(3-4): 337–347.

Application Data

WB

Validated by Selleck