Flotillin-2 Rabbit Recombinant mAb Datasheet

生物描述

特异性	Flotillin-2 Rabbit Recombinant mAb detects endogenous level of total Flotillin-2.
背景	Flotillin 1 and flotillin 2 are membrane-associated proteins that are thought to function in a number of cellular contexts, including signalling, endocytosis and interactions with the cytoskeleton. Flotillins are associated with the cytosolic side of the plasma membrane, but are also present intracellularly in different vesicular compartments like endosomes, lysosomes and phagolysosomes. In mammals, flotillin-2 is widely expressed in numerous different tissues, while flotillin-1 appears to be more restricted. Flotillin-1 is highly expressed in brain, heart, placenta and hematopoietic cells. In contrast, PCR analysis of flotillin-2 in murine and human tissues showed ubiquitous expression. It is known that the expression of flotillins varies in different cell types and tissues. As in many other cell types in the human body, flotillin-2 was clearly expressed in CaCo-2w cells, being model cells of human enterocytes. There, flotillin-2 was distinctly found in association with the plasma membrane, but also to a high degree in association with intracellular vesicles.

特异性

Flotillin-2 Rabbit Recombinant mAb detects endogenous level of total Flotillin-2.

背景

Flotillin 1 and flotillin 2 are membrane-associated proteins that are thought to function in a number of cellular contexts, including signalling, endocytosis and interactions with the cytoskeleton. Flotillins are associated with the cytosolic side of the plasma membrane, but are also present intracellularly in different vesicular compartments like endosomes, lysosomes and phagolysosomes. In mammals, flotillin-2 is widely expressed in numerous different tissues, while flotillin-1 appears to be more restricted. Flotillin-1 is highly expressed in brain, heart, placenta and hematopoietic cells. In contrast, PCR analysis of flotillin-2 in murine and human tissues showed ubiquitous expression. It is known that the expression of flotillins varies in different cell types and tissues. As in many other cell types in the human body, flotillin-2 was clearly expressed in CaCo-2w cells, being model cells of human enterocytes. There, flotillin-2 was distinctly found in association with the plasma membrane, but also to a high degree in association with intracellular vesicles.

使用信息

抗体应用

WB,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

47kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck