Datasheet

生物描述

特异性	GABA A Receptor alpha 5 Rabbit Recombinant mAb detects endogenous levels of total GABA A Receptor alpha 5
背景	γ-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the brain and is responsible for regulating neuronal excitability. There are at least three different receptors that it targets — GABAA, GABAB and GABAC. The GABAA receptors are multisubunit proteins. The subunits of rat GABAA receptors have been localized in the central nervous system at the protein level using subunit-specific antibodies. Outside the central nervous system, GABAA receptors have been found in the liver, in smooth airway muscles of the lung, and in several types of immune cells. They are generally GABA-gated anion channels selective for Cl− ions, with some permeability for bicarbonate anions. Benzodiazepines are positive modulators at GABAA receptors and exert their effects by binding to the interface between the γ2 and α subunits on the GABAA receptor. They only bind to the receptors containing the α5, α3, α2, and α1 subunits. Each of these subunits mediates different effects. In knockout mice, the α1 subunit has shown hypnotic/sedative effects, whilst the α3, α2 and α5 subunits have anti-anxiolytic effects and these have been exploited for therapeutic use. Selective GABRA5 negative modulators have nootropic effects without any serious side effects, which are seen in non-selective negative modulators at the alpha subunit of the GABAA receptor. GABRA5 negative modulators could be used to treat Alzheimer's disease while GABRA5 does not improve age-related cognitive defects.

特异性

GABA A Receptor alpha 5 Rabbit Recombinant mAb detects endogenous levels of total GABA A Receptor alpha 5

背景

γ-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the brain and is responsible for regulating neuronal excitability. There are at least three different receptors that it targets — GABAA, GABAB and GABAC. The GABAA receptors are multisubunit proteins. The subunits of rat GABAA receptors have been localized in the central nervous system at the protein level using subunit-specific antibodies. Outside the central nervous system, GABAA receptors have been found in the liver, in smooth airway muscles of the lung, and in several types of immune cells. They are generally GABA-gated anion channels selective for Cl− ions, with some permeability for bicarbonate anions. Benzodiazepines are positive modulators at GABAA receptors and exert their effects by binding to the interface between the γ2 and α subunits on the GABAA receptor. They only bind to the receptors containing the α5, α3, α2, and α1 subunits. Each of these subunits mediates different effects. In knockout mice, the α1 subunit has shown hypnotic/sedative effects, whilst the α3, α2 and α5 subunits have anti-anxiolytic effects and these have been exploited for therapeutic use. Selective GABRA5 negative modulators have nootropic effects without any serious side effects, which are seen in non-selective negative modulators at the alpha subunit of the GABAA receptor. GABRA5 negative modulators could be used to treat Alzheimer's disease while GABRA5 does not improve age-related cognitive defects.

使用信息

抗体应用

wb,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

70kDa

抗体类型

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck