GluR2 Rabbit Recombinant mAb Datasheet

生物描述

特异性	GluR2 Rabbit Recombinant mAb detects endogenous level of total GluR2.
背景	In the mammalian central nervous system, AMPA-type glutamate receptors mediate the vast majority of fast excitatory synaptic transmission. AMPARs are tetramers made up of combinations of four subunits: GluR1, GluR2, GluR3, and GluR4. AMPARs are widely expressed throughout the central nervous system both in neurons and in glia. The great majority of AMPARs in the central nervous system exist as heteromers containing GluR2. During early postnatal development, expression of GluR2 is low compared with that of GluR1, but it increases rapidly during the first postnatal week. GluR2 is a critical subunit in determining mammalian AMPAR function. This subunit determines many of the major biophysical properties of the native receptor, including, but not limited to, receptor kinetics, single-channel conductance, Ca2+ permeability, and block by endogenous polyamines. In addition, it is the most tightly regulated of the glutamate receptor subunits, with a number of specific regulatory processes at the level of gene expression, RNA editing, receptor assembly, and trafficking. It is of critical importance for normal brain function. GluR2 subunit dictates the critical biophysical properties of the receptor, strongly influences receptor assembly and trafficking, and plays pivotal roles in a number of forms of long-term synaptic plasticity.

特异性

GluR2 Rabbit Recombinant mAb detects endogenous level of total GluR2.

背景

In the mammalian central nervous system, AMPA-type glutamate receptors mediate the vast majority of fast excitatory synaptic transmission. AMPARs are tetramers made up of combinations of four subunits: GluR1, GluR2, GluR3, and GluR4. AMPARs are widely expressed throughout the central nervous system both in neurons and in glia. The great majority of AMPARs in the central nervous system exist as heteromers containing GluR2. During early postnatal development, expression of GluR2 is low compared with that of GluR1, but it increases rapidly during the first postnatal week. GluR2 is a critical subunit in determining mammalian AMPAR function. This subunit determines many of the major biophysical properties of the native receptor, including, but not limited to, receptor kinetics, single-channel conductance, Ca2+ permeability, and block by endogenous polyamines. In addition, it is the most tightly regulated of the glutamate receptor subunits, with a number of specific regulatory processes at the level of gene expression, RNA editing, receptor assembly, and trafficking. It is of critical importance for normal brain function. GluR2 subunit dictates the critical biophysical properties of the receptor, strongly influences receptor assembly and trafficking, and plays pivotal roles in a number of forms of long-term synaptic plasticity.

使用信息

抗体应用

WB,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

98kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Isaac JT, et al. Neuron. 2007, 54(6):859-71.

Application Data

WB

Validated by Selleck