Datasheet

生物描述

特异性	GRP94 Rabbit Recombinant mAb detects endogenous levels of total GRP94.
背景	Grp94, also known as GP96 or endoplasmin, is the Hsp90 paralog of the endoplasmic reticulum and plays a crucial role in protein secretion. Grp94 is only found in higher eukaryotes and is a dimeric protein. The most important activity of GRP94 is as a molecular chaperone that directs folding and/or assembly of secreted and membrane proteins. It seems important for the maturation of a number of client proteins, such as integrins, immunoglobulins, and Toll-like receptors. Together with BiP/GRP78, GRP94 is probably best known as the hallmark of the UPR response. It is a glucose responsive protein whose transcription and translation are induced when the cellular supply of glucose diminishes below physiological levels. When glucose is low within physiological levels, GRP94 expression is actually downregulated. GRP94 also responds in the same manner to all types of stresses that affect the ER machinery -- perturbations of calcium or redox homeostasis, inhibition of glycosylation, amino acid analogs that likely cause nascent chain misfolding, hypoxia, etc. However, unlike HSP90α and β, GRP94 is not induced by high temperature or other stresses that are unique to the cytosol. The main mechanism controlling the expression of GRP94 is transcriptional regulation. Transcription of GRP94 is usually initiated by activation of the signal transduction pathways that inform on changes in physiological conditions in the ER lumen and cause the activation of transcription factors - the Ire-1/XBP-1 pathway, the PERK/eIF2a pathway and the ATF6 pathway.

特异性

GRP94 Rabbit Recombinant mAb detects endogenous levels of total GRP94.

背景

Grp94, also known as GP96 or endoplasmin, is the Hsp90 paralog of the endoplasmic reticulum and plays a crucial role in protein secretion. Grp94 is only found in higher eukaryotes and is a dimeric protein. The most important activity of GRP94 is as a molecular chaperone that directs folding and/or assembly of secreted and membrane proteins. It seems important for the maturation of a number of client proteins, such as integrins, immunoglobulins, and Toll-like receptors. Together with BiP/GRP78, GRP94 is probably best known as the hallmark of the UPR response. It is a glucose responsive protein whose transcription and translation are induced when the cellular supply of glucose diminishes below physiological levels. When glucose is low within physiological levels, GRP94 expression is actually downregulated. GRP94 also responds in the same manner to all types of stresses that affect the ER machinery -- perturbations of calcium or redox homeostasis, inhibition of glycosylation, amino acid analogs that likely cause nascent chain misfolding, hypoxia, etc. However, unlike HSP90α and β, GRP94 is not induced by high temperature or other stresses that are unique to the cytosol. The main mechanism controlling the expression of GRP94 is transcriptional regulation. Transcription of GRP94 is usually initiated by activation of the signal transduction pathways that inform on changes in physiological conditions in the ER lumen and cause the activation of transcription factors - the Ire-1/XBP-1 pathway, the PERK/eIF2a pathway and the ATF6 pathway.

使用信息

抗体应用

WB, IHC, IF,ELISA,ELISA

稀释比例

WB	IHC	IF
1:1000	1:50	1:50

反应性

Human Mouse

MW (kDa)

94kDa

抗体类型

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck

IHC

Validated by Selleck

IF

Validated by Selleck