HAPLN1 Rabbit Recombinant mAb

生物描述

特异性

HAPLN1 Rabbit Recombinant mAb detects endogenous level of HAPLN1.

背景

Hyaluronan and proteoglycan link protein 1 (HAPLN1), also called cartilage link protein 1 (CRTL1), is a key component of the cartilage extracellular matrix. HAPLN1 stabilizes aggregates of aggrecan and hyaluronic acid by binding to aggrecan along the hyaluronic acid chain. The resulting aggregates have a stable macromolecular structure that contributes to compression resistance and shock absorption in the joints. In addition, a synthetic peptide of HAPLN1 stimulates the biosynthesis of collagens II, IX, and proteoglycan in the intervertebral disc cells. HAPLN1 may function as a growth factor that upregulates the synthesis of aggrecan and type II collagen in cartilage. HAPLN1 mRNA expression is upregulated by SOX9, a key regulator of cartilage matrix genes and chondrogenesis, and is downregulated in osteoarthritic cartilage. Thus, HAPLN1 seems to be an important regulator of cartilage homeostasis and may contribute to the pathogenesis of osteoarthritis with spinal degeneration.

使用信息

抗体应用	WB, IHC, IF,ELISA,ELISA
稀释比例	WB IHC IF 1:1000 1:50 1:50
反应性	Human Mouse Rat
MW (kDa)	48kDa
抗体类型	Rabbit
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Tomohiko Urano, et al. Eur Spine J. 2011, 20(4): 572–577.

Application Data

WB

IHC

IF