生物描述

特异性

HDAC1 Rabbit Recombinant mAb detects endogenous levels of total HDAC1.

背景

Histone deacetylases (HDACs) are a family of proteins that are able to deacetylate lysine residues on histones, thus resulting in the compaction of chromatin into a transcriptionally repressed state. HDACs also deacetylate a variety of other nuclear, cytoplasmic, and mitochondrial proteins, thereby regulating diverse cellular events. Vertebrates express 18 HDACs that are organized into four classes based on their similarity to yeast proteins: the Class I RPD3-like proteins (HDAC1, HDAC2, HDAC3, and HDAC8), the Class II HDA1-like proteins (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9, HDRP, and HDAC10), the Class III SIR2-like proteins (SIRT1–7), and HDAC11, which shares sequence homology with both Class I and Class II HDACs and is the sole member of the Class IV subfamily. Although Class I, II, and IV HDACs are zinc-dependent enzymes collectively referred to as classical HDACs, Class III HDACs require NAD+ for their activity and are referred to as sirtuins. HDAC1 and its closely related protein HDAC2 are often present in repressor complexes, such as Sin3, NuRD and CoREST complexes. HDAC1 can undergo post transcription modifications, such as phosphorylation, sumoylation and acetylation. Acetylated HDAC1 lost deacetylase activity. Acetylated HDAC1 also inhibit the deacetylase activity of HDAC2, hence to down regulate the overall deacetylase activity of HDAC1/2 containing complexes.

使用信息

抗体应用	WB, IF,ELISA,ELISA
稀释比例	WB IF 1:1000 1:50
反应性	Human Mouse Rat
MW (kDa)	55kDa
抗体类型
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Bardai FH, et al. J Biol Chem. 2012, 287(42):35444-53. [2] Tao Yang, et al. Blood. 2010, 116:3869.

Application Data

WB

IF