生物描述

特异性

HP1 gamma Rabbit Recombinant mAb detects endogenous levels of total HP1 gamma.

背景

Heterochromatin protein 1 (HP1) consists of highly conserved proteins, which have important functions in the cell nucleus including gene repression by heterochromatin formation, transcriptional activation, regulation of binding of cohesion complexes to centromeres, sequesteration of genes to nuclear periphery, transcriptional arrest, maintenance of heterochromatin integrity, gene repression at the single nucleosome level, gene repression by heterochromatization of euchromatin and DNA repair. HP1 could be also found in euchromatic sites, exerting gene repression activity. Mammals harbor three HP1 isotypes termed HP1α, HP1β, and HP1γ. HP1γ binds to di- or trimethylated lysine 9 on histone H3 (H3K9) and plays an important role in chromatin packaging and gene transcriptional regulation. HP1γ plays an important role in promoting NSCLC and may represent a novel prognostic biomarker and therapeutic target for the disease.

使用信息

抗体应用	WB, IHC, IF,ELISA,ELISA
稀释比例	WB IHC IF 1:1000 1:50 1:50
反应性	Human Mouse Rat
MW (kDa)	24kDa
抗体类型
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] James TC, et al. Mol Cell Biol. 1986, 6(11):3862-72. [2] Eissenberg JC, et al. Proc Natl Acad Sci U S A. 1990, 87(24):9923-7. [3] Zhou J, et al. Tumour Biol. 2014, 35(10):9777-85.

Application Data

WB

IHC

IF