生物描述

特异性

Huntingtin Rabbit Recombinant mAb detects endogenous levels of total Huntingtin.

背景

Huntington's Disease (HD) is a progressive and disabling neurodegenerative disorder of the central nervous system. This disease is caused by inheritance of an autosomal dominant mutation in the Huntingtin (Htt) protein. Huntingtin (Htt) is widely expressed during development and has a complex and dynamic distribution within cells. It is predicted to be a protein of pleiotropic function, interacting with a large number of effector proteins to mediate a host of physiological processes. A large number of Htt protein interactors function in microtubule-based axon trafficking. Huntingtin-associated protein 1 (HAP1) helps mediate the interaction between Htt, microtubule motor proteins and their co-factors, including kinesin, dynactin, and dynein. Huntingtin is involved in both antero- and retrograde axon transport. Htt associates with the endocytosis proteins clathrin and dynamin, as well as endocytic organelle trafficking proteins such as Endophilin 3, α-Adaptin, HIP-14, HAP1, and Huntingtin-associated protein 40 (HAP40). Htt is also associated with the movement of mitochondria along neurites and has neuroprotective functions during numerous proapoptotic challenges.

使用信息

抗体应用	WB, IHC,ELISA,ELISA
稀释比例	WB IHC 1:1000 1:50
反应性	Human Mouse Rat
MW (kDa)	348kDa
抗体类型
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Joost Schulte, et al. Curr Trends Neurol. 2011, 5: 65–78.

Application Data

WB

IHC