生物描述

特异性

HuR/ELAVL1 Rabbit Recombinant mAb detects endogenous levels of total HuR/ELAVL1.

背景

Hu antigen R (HuR, ELAVL1), a RNA-binding protein, is a member of the embryonic lethal, abnormal vision, Drosophila-like (ELAVL) family. In addition to HuA (HuR, ELAVL1), This family also includes HuB (Hel-N1, ELAVL2), HuC (ELAVL3), and HuD (ELAVL4). Expression of HuB, HuC, and HuD proteins is limited to neuronal tissues/cells, whereas HuR is ubiquitously expressed. HuR is predominantly nuclear but shuttles between the nucleus and the cytoplasm via a nucleocytoplasmic shuttling domain. This process is facilitated by the interaction of this domain with transport adaptors like pp32 and APRIL or directly with transport receptors such as CRM1 for nuclear export and transportins 1 and 2 (TRN1/2) for nuclear import. HuR has been shown to modulate a variety of post-transcriptional decisions in several key cellular processes, including immune response, exposure to stressing agents, cell survival, inflammation, cell cycle control, differentiation program, oncogenesis, and replicative senescence.

使用信息

抗体应用	WB, IF,ELISA,ELISA
稀释比例	WB IF 1:1000 1:50
反应性	Human Mouse Rat
MW (kDa)	36kDa
抗体类型
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Izquierdo JM, et al. J Biol Chem. 2008, 283(27):19077-84. [2] Katsanou V, et al. Mol Cell Biol. 2009, 29(10):2762-76.

Application Data

WB

IF