IDH2 Rabbit Recombinant mAb

生物描述

特异性

IDH2 Rabbit Recombinant mAb detects endogenous level of total IDH2.

背景

Isocitrate dehydrogenases (IDHs) catalyze the oxidative decarboxylation of isocitrate to α-ketoglutarate and reduce NAD(P)+ to NAD(P)H. IDH2 contains an N-terminal mitochondrial signal peptide and localizes to the mitochondria. It is highly expressed in mammalian heart, muscle, and activated lymphocytes and moderately expressed elsewhere. IDH2 plays prominent yet distinctive roles in cellular metabolism and is involved in the regulation of oxidative respiration. IDH2 plays a key role in TCA cycle regulation in multiple tissues. IDH2 is a protector against various insults and plays a crucial role in the defense against oxidative stress. Lipid peroxidation products, singlet oxygen, hypochlorous acid, ROS, nitric oxide, and peroxynitrite can inactivate IDH2. Frequent mutations of IDH2 are found in human diseases, especially cancers.

使用信息

抗体应用	WB, IHC,ELISA,ELISA
稀释比例	WB IHC 1:1000 1:50
反应性	Human Mouse Rat
MW (kDa)	45kDa
抗体类型	Rabbit
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Zachary J. Reitman, et al. J Natl Cancer Inst. 2010, 102(13): 932-941.

Application Data

WB

IHC