IRAK3 Rabbit Recombinant mAb Datasheet

生物描述

特异性	IRAK3 Rabbit Recombinant mAb detects endogenous level of total IRAK3.
背景	IL-1 receptor-associated kinases (IRAK) family is comprised of four family members, IRAK-1, IRAK-2, IRAK-3 (also known as IRAK-M), and IRAK-4, which play important roles in both positively and negatively regulating the expression of inflammatory molecules. These signals are critical for elimination of viruses, bacteria, and cancer cells, as well as for wound healing. Similar to the other IRAK proteins, IRAK-3 (a.k.a. IRAK-M) can form complexes with MyD88 and TRAF-6, Like IRAK-2, it is considered to be a pseudokinase with very limited capacity for auto-phosphorylation, but with the potential to become activated by other IRAK proteins and serve as a functional kinase. In contrast to other IRAK proteins, IRAK-M is thought to function as a negative regulator that prevents the dissociation of IRAK-1 and IRAK-2 from the receptor complex, inhibiting their interaction with TRAF-6 and interrupting the downstream inflammatory cascade. Unlike other IRAK family members that are widely expressed on a variety of cell types, IRAK-M is thought to chiefly reside in monocyte and macrophage populations.

特异性

IRAK3 Rabbit Recombinant mAb detects endogenous level of total IRAK3.

背景

IL-1 receptor-associated kinases (IRAK) family is comprised of four family members, IRAK-1, IRAK-2, IRAK-3 (also known as IRAK-M), and IRAK-4, which play important roles in both positively and negatively regulating the expression of inflammatory molecules. These signals are critical for elimination of viruses, bacteria, and cancer cells, as well as for wound healing. Similar to the other IRAK proteins, IRAK-3 (a.k.a. IRAK-M) can form complexes with MyD88 and TRAF-6, Like IRAK-2, it is considered to be a pseudokinase with very limited capacity for auto-phosphorylation, but with the potential to become activated by other IRAK proteins and serve as a functional kinase. In contrast to other IRAK proteins, IRAK-M is thought to function as a negative regulator that prevents the dissociation of IRAK-1 and IRAK-2 from the receptor complex, inhibiting their interaction with TRAF-6 and interrupting the downstream inflammatory cascade. Unlike other IRAK family members that are widely expressed on a variety of cell types, IRAK-M is thought to chiefly reside in monocyte and macrophage populations.

使用信息

抗体应用

WB, IHC,ELISA,ELISA

稀释比例

WB	IHC
1:1000	1:50

反应性

Human

MW (kDa)

68kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Ajay Jain, et al. Front Immunol. 2014, 5: 553.

Application Data

WB

Validated by Selleck

IHC

Validated by Selleck