IRAK4 Rabbit Recombinant mAb Datasheet

生物描述

特异性	IRAK4 Rabbit Recombinant mAb detects endogenous level of total IRAK4.
背景	IL-1 receptor-associated kinases (IRAK) family is comprised of four family members, IRAK-1, IRAK-2, IRAK-3 (also known as IRAK-M), and IRAK-4, which play important roles in both positively and negatively regulating the expression of inflammatory molecules. These signals are critical for elimination of viruses, bacteria, and cancer cells, as well as for wound healing. Of the four IRAK proteins, IRAK-1 and IRAK-4 are active serine/threonine kinases. IRAK-4, the most recent IRAK family protein to be discovered, is the most proximal IRAK family protein in the TIR-mediated signaling pathway and directly downstream of MyD88. IRAK-4 and IRAK-1 are able to associate with each other upon engaging MyD88 through their DD. IRAK-4 is thought to phosphorylate IRAK-1, which allows IRAK-1 to initiate an auto-phosphorylation cascade occurring in three sequential steps. It is considered the “master IRAK” because it is required for all MyD88-dependent NF-κB activation and for inducing IFNα expression through TLR 7, 8, and 9.

特异性

IRAK4 Rabbit Recombinant mAb detects endogenous level of total IRAK4.

背景

IL-1 receptor-associated kinases (IRAK) family is comprised of four family members, IRAK-1, IRAK-2, IRAK-3 (also known as IRAK-M), and IRAK-4, which play important roles in both positively and negatively regulating the expression of inflammatory molecules. These signals are critical for elimination of viruses, bacteria, and cancer cells, as well as for wound healing. Of the four IRAK proteins, IRAK-1 and IRAK-4 are active serine/threonine kinases. IRAK-4, the most recent IRAK family protein to be discovered, is the most proximal IRAK family protein in the TIR-mediated signaling pathway and directly downstream of MyD88. IRAK-4 and IRAK-1 are able to associate with each other upon engaging MyD88 through their DD. IRAK-4 is thought to phosphorylate IRAK-1, which allows IRAK-1 to initiate an auto-phosphorylation cascade occurring in three sequential steps. It is considered the “master IRAK” because it is required for all MyD88-dependent NF-κB activation and for inducing IFNα expression through TLR 7, 8, and 9.

使用信息

抗体应用

WB, IHC, IF,ELISA,ELISA

稀释比例

WB	IHC	IF
1:1000	1:50	1:50

反应性

Human

MW (kDa)

52kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Ajay Jain, et al. Front Immunol. 2014, 5: 553.

Application Data

WB

Validated by Selleck

IHC

Validated by Selleck

IF

Validated by Selleck