IRF3 Rabbit Recombinant mAb

生物描述

特异性

IRF3 Rabbit Recombinant mAb detects endogenous level of total IRF3.

背景

Interferon regulatory factor (IRF)3 is critical for the transcriptional induction of chemokines and cytokines during viral or bacterial invasion. IRF3 is constitutively expressed in two forms (forms I and II) and localizes in the cytoplasm in unstimulated cells. On viral infection, IRF3 is phosphorylated within the C-terminus of the protein on serine 385, 386, 396, 398, 402, 405, and threonine 404, resulting in hyper-phosphorylated forms III and IV. It was reported that C-terminal phosphorylation may elicit a conformational change that allowed the formation of the IRF3 dimer, which translocated to the nucleus and formed a transcriptional complex with the coactivator CBP/p300. This ultimately induces the early production of antiviral proteins (e.g., IFN-β) and is critical for establishing an antiviral state in host cells.

使用信息

抗体应用	WB, IHC,ELISA,ELISA
稀释比例	WB IHC 1:1000 1:50
反应性	Human Mouse Rat
MW (kDa)	57kDa
抗体类型	Rabbit
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Zhang B, et al. Cell Res. 2009, 19(4):412-28. [2] Shi HX, et al. Mol Cell Biol. 2010, 30(10):2424-36.

Application Data

WB

IHC