ITCH Rabbit Recombinant mAb Datasheet

生物描述

特异性	ITCH Rabbit Recombinant mAb detects endogenous level of total ITCH.
背景	Itch is a monomeric protein, which belongs to the homologous to E6-AP carboxy terminus (HECT)-type family of E3s, whose modular structural organization consists of an N-terminal Ca2+-dependent phospholipid-binding C2 domain, multiple protein-protein interaction WW domains, and a C-terminal HECT domain. Although a small fraction of Itch displays a perinuclear distribution overlapping with the trans-Golgi network, the protein is predominantly associated with early and late endosomal compartments and lysosomes. Localization of Itch to the endocytic vesicles is mediated by the C2 domain. Itch typically regulates the stability of both transmembrane receptors through canonical monoubiquitylation or multiubiquitylation, and intracellular substrates through polyubiquitylation, driving them to lysosomal and proteasomal degradation, respectively. Proteolysis-independent ubiquitylation events have been also ascribed to the E3 activity of Itch. The ubiquitin ligase Itch plays key roles in different cellular contexts, in virtue of its functionally distinct substrates. A significant number of the transcriptional regulators targeted by Itch for proteasomal destruction are crucially involved in controlling cell growth, differentiation, and apoptotic processes.

特异性

ITCH Rabbit Recombinant mAb detects endogenous level of total ITCH.

背景

Itch is a monomeric protein, which belongs to the homologous to E6-AP carboxy terminus (HECT)-type family of E3s, whose modular structural organization consists of an N-terminal Ca2+-dependent phospholipid-binding C2 domain, multiple protein-protein interaction WW domains, and a C-terminal HECT domain. Although a small fraction of Itch displays a perinuclear distribution overlapping with the trans-Golgi network, the protein is predominantly associated with early and late endosomal compartments and lysosomes. Localization of Itch to the endocytic vesicles is mediated by the C2 domain. Itch typically regulates the stability of both transmembrane receptors through canonical monoubiquitylation or multiubiquitylation, and intracellular substrates through polyubiquitylation, driving them to lysosomal and proteasomal degradation, respectively. Proteolysis-independent ubiquitylation events have been also ascribed to the E3 activity of Itch. The ubiquitin ligase Itch plays key roles in different cellular contexts, in virtue of its functionally distinct substrates. A significant number of the transcriptional regulators targeted by Itch for proteasomal destruction are crucially involved in controlling cell growth, differentiation, and apoptotic processes.

使用信息

抗体应用

WB,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

103kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Melino G, et al. Cell Death Differ. 2008, 15(7):1103-12.

Application Data

WB

Validated by Selleck