生物描述

特异性

KAT7/HBO1/MYST2 Rabbit Recombinant mAb detects endogenous levels of total KAT7/HBO1/MYST2.

背景

The MYST protein family are catalytic subunits of histone acetyltransferase (HAT) complexes. The MYST protein family are catalytic subunits of histone acetyltransferase (HAT) complexes. The MYST2 HAT is a major mediator of both histone H3 (K14, K23) and H4 (K5, K8, K12) acetylation in vivo. MYST2/HBO1 associates in a combinatorial manner with paralogs JADE1-2-3, the human tumour suppressor proteins ING4 and ING5 and EAF6 in a complex responsible for the majority of histone H4 acetylation in higher eukaryotes. Certain roles of MYST2 are independent of histone acetylation and accordingly MYST2 is known to acetylate several substrates other than histones. Acetylation of non-histone substrates can have an effect on their stability, activity, localisation or interaction with other proteins. MYST2 has been shown to associate with and acetylate several pre-replicative complex subunits, namely ORC1, ORC2, MCM2, CDC6 and geminin, and indeed MYST2 has a role not only in replication licensing through the regulation of pre-replicative complex assembly, but possibly also in the activation of origins of replication and in replication fork movement.

使用信息

抗体应用	WB, IF,ELISA
稀释比例	WB IF 1:1000 1:50
反应性	Human Mouse Rat
MW (kDa)	75kDa
抗体类型
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Ohzeki J, et al. Dev Cell. 2016, 37(5):413-27. [2] Mercedes Pardo, et al. Scientific Reports. 2017, 7, Article number: 8157.

Application Data

WB

IF